Comparative Studies for Cryopreservation of Agave Shoot Tips by Droplet-Vitrification.

Autor:	Delgado-Aceves L; Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Unidad de Biotecnología Vegetal, Zapopan 45019, Jalisco, Mexico., Corona S; Departamento de Botánica y Zoología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopan 44600, Jalisco, Mexico., Marin-Castro UR; Facultad de Ciencias Químicas, Universidad Veracruzana, Xalapa 91000, Veracruz, Mexico., Rascón-Díaz MP; Centro de Investigación y Desarrollo en Alimentos, Xalapa 91190, Veracruz, Mexico., Portillo L; Departamento de Botánica y Zoología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopan 44600, Jalisco, Mexico., Gutiérrez-Mora A; Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Unidad de Biotecnología Vegetal, Zapopan 45019, Jalisco, Mexico., González-Arnao MT; Facultad de Ciencias Químicas, Universidad Veracruzana, Orizaba 94340, Veracruz, Mexico.
Jazyk:	angličtina
Zdroj:	Plants (Basel, Switzerland) [Plants (Basel)] 2024 Sep 18; Vol. 13 (18). Date of Electronic Publication: 2024 Sep 18.
DOI:	10.3390/plants13182609
Abstrakt:	The objective of this work was to assess the suitability of the Droplet-vitrification protocol previously developed with Agave peacockii shoot tips for the cryopreservation of six Agave species. Shoot tips were precultured for 1 day on a medium with 0.3 M sucrose in the dark, loaded in a solution with 1.6 M glycerol and 0.4 M sucrose for 20 min, and dehydrated by exposure to Plant Vitrification Solution 2 (PVS2) at 0 °C for 20 min. Complementary studies using histological analysis, Differential scanning calorimetry (DSC), and evaluation of morphological characteristics in cryo-derived plants were performed. Survival rates ranged from 84% to 100% and from 76% to 97% before and after cryopreservation regardless of the Agave species belonging to two taxonomic subgenera. Thermal analysis of shoot tips subjected to the successive steps of the Droplet-vitrification protocol identified ice crystal formation after loading treatment and glass transition after osmotic dehydration with PVS2. The average glass transition temperature (Tg) was -55.44 °C based on the results of four Agave species. The histological studies showed the anatomical differences that could be found in the meristematic structures depending on the loss of apical dominance. This is the most advanced research on cryopreservation of Agave shoot tips.
Databáze:	MEDLINE
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