Genetic Diversity of Common Bean ( Phaseolus vulgaris L.) Landraces Based on Morphological Traits and Molecular Markers.

Autor: de Paula E; Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural (INCAPER), 60 Kurt Lwein Av., Domingos Martins 29273-700, ES, Brazil., Almeida RN; Laboratory of Agricultural Engineering, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 2000 Alberto Lamego Av., Campos dos Goytacazes 28013-602, RJ, Brazil., Santos TO; Laboratory of Genetics and Plant Breeding, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 2000 Alberto Lamego Av., Campos dos Goytacazes 28013-602, RJ, Brazil., Souza Neto JD; Instituto Federal de Educação, Ciência e Tecnologia do Espírito Santo-Campus de Alegre, Rodovia ES-482 (Cachoeiro-Alegre, Km 72), Alegre 29500-000, ES, Brazil., Riva-Souza EM; Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural (INCAPER), 60 Kurt Lwein Av., Domingos Martins 29273-700, ES, Brazil., Posse SCP; Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural (INCAPER), 60 Kurt Lwein Av., Domingos Martins 29273-700, ES, Brazil., Souza MN; Instituto Federal de Educação, Ciência e Tecnologia do Espírito Santo-Campus de Alegre, Rodovia ES-482 (Cachoeiro-Alegre, Km 72), Alegre 29500-000, ES, Brazil., Madella de Oliveira AF; Instituto Federal de Educação, Ciência e Tecnologia do Espírito Santo-Campus de Alegre, Rodovia ES-482 (Cachoeiro-Alegre, Km 72), Alegre 29500-000, ES, Brazil., Santos Júnior AC; Instituto Federal de Educação, Ciência e Tecnologia do Espírito Santo-Campus de Alegre, Rodovia ES-482 (Cachoeiro-Alegre, Km 72), Alegre 29500-000, ES, Brazil., Santos JO; Department of Biology, Universidade Federal do Piauí, 1401-1519 Dirce Oliveira St., Teresina 64048-550, PI, Brazil., Pimenta S; Department of Agriculture, Universidade Estadual de Montes Claros (UNIMONTES), 2630 Reinaldo Viana St., Janaúba 39440-000, MG, Brazil., Bento CDS; Laboratory of Plant Breeding, Universidade Federal do Espírito Santo (UFES), Alto Universitário, Alegre 29500-000, ES, Brazil., Moulin MM; Instituto Federal de Educação, Ciência e Tecnologia do Espírito Santo-Campus de Alegre, Rodovia ES-482 (Cachoeiro-Alegre, Km 72), Alegre 29500-000, ES, Brazil.
Jazyk: angličtina
Zdroj: Plants (Basel, Switzerland) [Plants (Basel)] 2024 Sep 15; Vol. 13 (18). Date of Electronic Publication: 2024 Sep 15.
DOI: 10.3390/plants13182584
Abstrakt: The objective of this study was to evaluate the genetic diversity among traditional common bean accessions through morphological descriptors and molecular markers. Sixty-seven common bean accessions from the Germplasm bank of the Instituto Federal of Espírito Santo-Campus de Alegre were evaluated. For this, 25 specific morphological descriptors were used, namely 12 quantitative and 13 qualitative ones. A diversity analysis based on morphological descriptors was carried out using the Gower algorithm. For molecular characterization, 23 ISSR primers were used to estimate dissimilarity using the Jaccard Index. Based on the dendrograms obtained by the UPGMA method, for morphological and molecular characterization, high genetic variability was observed between the common bean genotypes studied, evidenced by cophenetic correlation values in the order of 0.99, indicating an accurate representation of the dissimilarity matrix by the UPGMA clustering. In the morphological characterization, high phenotypic diversity was observed between the accessions, with grains of different shapes, colors, and sizes, and the accessions were grouped into nine distinct groups. Molecular characterization was efficient in separating the genotypes in the Andean and Mesoamerican groups, with the 23 ISSR primers studied generating an average of 6.35 polymorphic bands. The work identified divergent accessions that can serve different market niches, which can be indicated as parents to form breeding programs in order to obtain progenies with high genetic variability.
Databáze: MEDLINE