| Autor: |
Mora-Cabello R; Department of Organic Chemistry, Faculty of Sciences, University of Málaga, 29071 Málaga, Spain., Fuentes-Ríos D; Department of Organic Chemistry, Faculty of Sciences, University of Málaga, 29071 Málaga, Spain., Gago L; Institute of Biopathology and Regenerative Medicine (IBIMER), Biomedical Research Center (CIBM), 18100 Granada, Spain.; Instituto de Investigación Biosanitaria de Granada (Ibs.GRANADA), 18012 Granada, Spain.; Department of Anatomy and Embryology, University of Granada, 18071 Granada, Spain., Cabeza L; Institute of Biopathology and Regenerative Medicine (IBIMER), Biomedical Research Center (CIBM), 18100 Granada, Spain.; Instituto de Investigación Biosanitaria de Granada (Ibs.GRANADA), 18012 Granada, Spain.; Department of Anatomy and Embryology, University of Granada, 18071 Granada, Spain., Moscoso A; Department of Organic Chemistry, Faculty of Sciences, University of Málaga, 29071 Málaga, Spain., Melguizo C; Institute of Biopathology and Regenerative Medicine (IBIMER), Biomedical Research Center (CIBM), 18100 Granada, Spain.; Instituto de Investigación Biosanitaria de Granada (Ibs.GRANADA), 18012 Granada, Spain.; Department of Anatomy and Embryology, University of Granada, 18071 Granada, Spain., Prados J; Institute of Biopathology and Regenerative Medicine (IBIMER), Biomedical Research Center (CIBM), 18100 Granada, Spain.; Instituto de Investigación Biosanitaria de Granada (Ibs.GRANADA), 18012 Granada, Spain.; Department of Anatomy and Embryology, University of Granada, 18071 Granada, Spain., Sarabia F; Department of Organic Chemistry, Faculty of Sciences, University of Málaga, 29071 Málaga, Spain., López-Romero JM; Department of Organic Chemistry, Faculty of Sciences, University of Málaga, 29071 Málaga, Spain. |
| Abstrakt: |
Background/Objectives : Magnetic Fe 3 O 4 nanoparticles (MNPs) are becoming more important every day. We prepared MNPs in a simple one-step reaction by following the solvothermal method, assisted by azide and alkyne functionalized polyethylene glycol (PEG400) polymers, as well as by PEG6000 and the polyol β-cyclodextrin (βCD), which played a crucial role as electrostatic stabilizers, providing polymeric/polyol coatings around the magnetic cores. Methods : The composition, morphology, and magnetic properties of the nanospheres were analyzed using Transmission Electron and Atomic Force Microscopies (TEM, AFM), Nuclear Magnetic Resonance (NMR), X-ray Diffraction Diffractometry (XRD), Fourier-Transform Infrared Spectroscopy (FT-IR), Matrix-Assisted Laser Desorption/Ionization (MALDI) and Vibrating Sample Magnetometry (VSM). Results : The obtained nanoparticles (@Fe 3 O 4 -PEGs and @Fe 3 O 4 -βCD) showed diameters between 90 and 250 nm, depending on the polymer used and the Fe 3 O 4 ·6H 2 O precursor concentration, typically, 0.13 M at 200 °C and 24 h of reaction. MNPs exhibited superparamagnetism with high saturation mass magnetization at room temperature, reaching values of 59.9 emu/g (@Fe 3 O 4 -PEG6000), and no ferromagnetism. Likewise, they showed temperature elevation after applying an alternating magnetic field (AMF), obtaining Specific Absorption Rate (SAR) values of up to 51.87 ± 2.23 W/g for @Fe 3 O 4 -PEG6000. Additionally, the formed systems are susceptible to click chemistry, as was demonstrated in the case of the cannabidiol-propargyl derivative (CBD-Pro), which was synthesized and covalently attached to the azide functionalized surface of @Fe 3 O 4 -PEG400-N 3 . Prepared MNPs are highly dispersible in water, PBS, and citrate buffer, remaining in suspension for over 2 weeks, and non-toxic in the T84 human colon cancer cell line, Conclusions : indicating that they are ideal candidates for biomedical applications. |
