| Autor: |
Triantafyllopoulou E; Section of Pharmaceutical Technology, Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens (NKUA), Panepistimioupolis Zografou, 15771 Athens, Greece., Perinelli DR; School of Pharmacy, University of Camerino, Via Gentile III da Varano, 62032 Camerino, Italy., Forys A; Centre of Polymer and Carbon Materials, Polish Academy of Sciences, 41-819 Zabrze, Poland., Pantelis P; Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens (NKUA), 11527 Athens, Greece., Gorgoulis VG; Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens (NKUA), 11527 Athens, Greece.; Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece.; Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, UK.; Faculty Institute for Cancer Sciences, Manchester Academic Health Sciences Centre, University of Manchester, Manchester M20 4GJ, UK.; Faculty of Health and Medical Sciences, University of Surrey, Surrey GU2 7YH, UK., Lagopati N; Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece.; Laboratory of Biology, Department of Basic Medical Sciences, Medical School, National and Kapodistrian University of Athens (NKUA), 11527 Athens, Greece., Trzebicka B; Centre of Polymer and Carbon Materials, Polish Academy of Sciences, 41-819 Zabrze, Poland., Bonacucina G; School of Pharmacy, University of Camerino, Via Gentile III da Varano, 62032 Camerino, Italy., Valsami G; Section of Pharmaceutical Technology, Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens (NKUA), Panepistimioupolis Zografou, 15771 Athens, Greece., Pippa N; Section of Pharmaceutical Technology, Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens (NKUA), Panepistimioupolis Zografou, 15771 Athens, Greece., Pispas S; Theoretical and Physical Chemistry Institute, National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, 11635 Athens, Greece. |
| Abstrakt: |
Despite the appealing properties of random copolymers, the use of these biomaterials in association with phospholipids is still limited, as several aspects of their performance have not been investigated. The aim of this work is the formulation of lipid/random copolymer platforms and the comprehensive study of their features by multiple advanced characterization techniques. Both biomaterials are amphiphilic, including two phospholipids (1,2-dioctadecanoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)) and a statistical copolymer of oligo (ethylene glycol) methyl ether methacrylate (OEGMA) and 2-(diisopropylamino) ethyl methacrylate (DIPAEMA). We examined the design parameters, including the lipid composition, the % comonomer ratio, and the lipid-to-polymer ratio that could be critical for their behavior. The structures were also probed in different conditions. To the best of the authors' knowledge, this is the first time that P(OEGMA-co-DIPAEMA)/lipid hybrid colloidal dispersions have been investigated from a membrane mechanics, biophysical, and morphological perspective. Among other parameters, the copolymer architecture and the hydrophilic to hydrophobic balance are deemed fundamental parameters for the biomaterial co-assembly, having an impact on the membrane's fluidity, morphology, and thermodynamics. Exploiting their unique characteristics, the most promising candidates were utilized for methotrexate (MTX) loading to explore their encapsulation capability and potential antitumor efficacy in vitro in various cell lines. |
