| Autor: |
Hutu I; Horia Cernescu Research Unit, Faculty of Veterinary Medicine, University of Life Sciences 'Regele Mihai I', 300645 Timisoara, Romania., Boldura OM; Horia Cernescu Research Unit, Faculty of Veterinary Medicine, University of Life Sciences 'Regele Mihai I', 300645 Timisoara, Romania., Luca I; Horia Cernescu Research Unit, Faculty of Veterinary Medicine, University of Life Sciences 'Regele Mihai I', 300645 Timisoara, Romania., Pasca SA; Faculty of Veterinary Medicine, University of Life Sciences, 700490 Iasi, Romania., Dragoescu AA; Faculty of Agriculture, University of Life Sciences 'Regele Mihai I', 300645 Timisoara, Romania., Gros RV; Horia Cernescu Research Unit, Faculty of Veterinary Medicine, University of Life Sciences 'Regele Mihai I', 300645 Timisoara, Romania., Lungu BC; Horia Cernescu Research Unit, Faculty of Veterinary Medicine, University of Life Sciences 'Regele Mihai I', 300645 Timisoara, Romania., Călugăriță A; Horia Cernescu Research Unit, Faculty of Veterinary Medicine, University of Life Sciences 'Regele Mihai I', 300645 Timisoara, Romania., Baltă C; Aurel Ardelean Institute of Life Sciences, Vasile Goldis Western University of Arad, 310025 Arad, Romania., Mircu C; Horia Cernescu Research Unit, Faculty of Veterinary Medicine, University of Life Sciences 'Regele Mihai I', 300645 Timisoara, Romania., Stancu AC; Horia Cernescu Research Unit, Faculty of Veterinary Medicine, University of Life Sciences 'Regele Mihai I', 300645 Timisoara, Romania. |
| Abstrakt: |
Pathogenic Leptospira spp. causes leptospirosis in animals and humans globally, leading to systemic infections that can impact vital organs in affected animals. The purpose of this study was to evaluate kidney injury and to perform a retrospective analysis of leptospirosis infection in follow-up dog samples. The retrospective study collected epidemiological information obtained through paraclinical exams, immunohistochemistry (IHC), and molecular biology (qPCR) of cases from the Faculty of Veterinary Medicine from Timisoara between September 2016 and May 2023. No correlations were found between Leptospira infection and breed ( p = 0.714), gender or castration status ( p = 0.890), and anatomic pathology exam results ( p = 0.608). Significant associations were found in cases with high levels of azotemia ( p = 0.000) and immunological status (vaccinated vs. unvaccinated, p = 0.000), with the leptospirosis risk in unvaccinated animals calculated at OR = 85.500 (95%CI, 6.82-1071.26, p = 0.000). Retrospectively, leptospirosis was diagnosed in 27/65 cases (42%) using the IHC method, while the qPCR assay detected 29/65 cases (45%). This study demonstrates that qPCR is a robust and specific method for postmortem diagnosis of Leptospira spp. infection in dogs, offering higher specificity and reliability compared to traditional IHC methods, which showed 94.74% specificity in our study. |
