Occurrence of Uncultured Legionella spp. in Treated Wastewater Effluent and Its Impact on Human Health (SCA.Re.S Project).

Autor: De Giglio O; Interdisciplinary Department of Medicine, University of Bari Aldo Moro, Piazza G. Cesare 11, 70124 Bari, Italy., Diella G; Interdisciplinary Department of Medicine, University of Bari Aldo Moro, Piazza G. Cesare 11, 70124 Bari, Italy., Bagordo F; Department of Pharmacy-Pharmaceutical Sciences, University of Bari Aldo Moro, Via Orabona 4, 70125 Bari, Italy., Savino AF; Hygiene Section, Azienda Ospedaliero Universitaria Policlinico di Bari, Piazza Giulio Cesare 11, 70124 Bari, Italy., Calabrese A; National Research Council of Italy (CNR), Water Research Institute (IRSA), Via F. De Blasio, 5, 70132 Bari, Italy., Campanale M; Engeo soc. cop. a.r.l., 70122 Bari, Italy., Triggiano F; Interdisciplinary Department of Medicine, University of Bari Aldo Moro, Piazza G. Cesare 11, 70124 Bari, Italy., Apollonio F; Interdisciplinary Department of Medicine, University of Bari Aldo Moro, Piazza G. Cesare 11, 70124 Bari, Italy., Spagnuolo V; Interdisciplinary Department of Medicine, University of Bari Aldo Moro, Piazza G. Cesare 11, 70124 Bari, Italy.; Department of Precision and Regenerative Medicine and Ionian Area (DiMePre-J), University of Bari Aldo Moro, 70121 Bari, Italy., Lopuzzo M; Interdisciplinary Department of Medicine, University of Bari Aldo Moro, Piazza G. Cesare 11, 70124 Bari, Italy.; Department of Precision and Regenerative Medicine and Ionian Area (DiMePre-J), University of Bari Aldo Moro, 70121 Bari, Italy., Grassi T; Department of Experimental Medicine, University of Salento, Via Monteroni 165, 73100 Lecce, Italy., Caputo MC; National Research Council of Italy (CNR), Water Research Institute (IRSA), Via F. De Blasio, 5, 70132 Bari, Italy., Brigida S; Department of Experimental Medicine, University of Salento, Via Monteroni 165, 73100 Lecce, Italy., Valeriani F; Department of Movement, Human, and Health Sciences, University of Rome 'Foro Italico', 00135 Rome, Italy., Romano Spica V; Department of Movement, Human, and Health Sciences, University of Rome 'Foro Italico', 00135 Rome, Italy., Montagna MT; Interdisciplinary Department of Medicine, University of Bari Aldo Moro, Piazza G. Cesare 11, 70124 Bari, Italy.
Jazyk: angličtina
Zdroj: Pathogens (Basel, Switzerland) [Pathogens] 2024 Sep 12; Vol. 13 (9). Date of Electronic Publication: 2024 Sep 12.
DOI: 10.3390/pathogens13090786
Abstrakt: Wastewater treatment plants (WWTPs) provide optimal conditions for the environmental spread of Legionella . As part of the Evaluation of Sanitary Risk Related to the Discharge of Wastewater to the Ground (SCA.Re.S) project, this study was conducted to evaluate the presence of Legionella in WWTP effluent and in groundwater samples collected from two wells located downstream from the plant. The samples were analyzed to determine the concentrations of Legionella spp using the standard culture-based method and molecular techniques, followed by genomic sequencing analysis. Legionella was detected only with the molecular methods (except in one sample of effluent positive for L. pneumophila serogroup 6), which showed viable Legionella pneumophila and L. non- pneumophila through the use of free DNA removal solution in both the effluent and groundwater, with concentrations that progressively decreased downstream from the plant. Viable L. pneumophila appeared to be slightly more concentrated in warm months. However, no significant differences ( p ≥ 0.05) in concentrations between cold and warm months were observed. A genotypic analysis characterized the species present in the samples and found that uncultured Legionella spp, as yet undefined, constituted the prevalent species in all the samples (range 77.15-83.17%). WWTPs play an important role in the hygienic and sanitary quality of groundwater for different uses. The application of Legionella control systems during the purification of effluents is warranted to prevent possible outbreaks of legionellosis.
Databáze: MEDLINE
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