High-Risk HPV Detection in Paraffin-Embedded Tissue from Cervical Lesions.

Autor: Almeida M; Health Sciences Research Centre (CICS), Faculty of Health Sciences, University of Beira Interior (UBI), Avenida Infante D. Henrique, 6200-506 Covilhã, Portugal.; Department of Pathology, Ipatimup Diagnostics, Rua Júlio Amaral de Carvalho, 45, 4200-135 Porto, Portugal.; I3S-Instituto de Investigação e Inovação em Saúde, University of Porto, 4200-135 Porto, Portugal.; Clinical Academic Centre of Beiras (CACB), Edifício UBImedical, Estrada Municipal 506, 6200-284 Covilhã, Portugal., Caeiro V; Health Sciences Research Centre (CICS), Faculty of Health Sciences, University of Beira Interior (UBI), Avenida Infante D. Henrique, 6200-506 Covilhã, Portugal.; Cova da Beira Local Health Unit, Alameda Pêro da Covilhã, 6200-251 Covilhã, Portugal., Costa D; Health Sciences Research Centre (CICS), Faculty of Health Sciences, University of Beira Interior (UBI), Avenida Infante D. Henrique, 6200-506 Covilhã, Portugal., Silva L; Health Sciences Research Centre (CICS), Faculty of Health Sciences, University of Beira Interior (UBI), Avenida Infante D. Henrique, 6200-506 Covilhã, Portugal., Sousa C; Health Sciences Research Centre (CICS), Faculty of Health Sciences, University of Beira Interior (UBI), Avenida Infante D. Henrique, 6200-506 Covilhã, Portugal., Pestana P; Health Sciences Research Centre (CICS), Faculty of Health Sciences, University of Beira Interior (UBI), Avenida Infante D. Henrique, 6200-506 Covilhã, Portugal.; Clinical Academic Centre of Beiras (CACB), Edifício UBImedical, Estrada Municipal 506, 6200-284 Covilhã, Portugal.; Cova da Beira Local Health Unit, Alameda Pêro da Covilhã, 6200-251 Covilhã, Portugal., Campelos S; Department of Pathology, Ipatimup Diagnostics, Rua Júlio Amaral de Carvalho, 45, 4200-135 Porto, Portugal.; I3S-Instituto de Investigação e Inovação em Saúde, University of Porto, 4200-135 Porto, Portugal., Vale J; Department of Pathology, Ipatimup Diagnostics, Rua Júlio Amaral de Carvalho, 45, 4200-135 Porto, Portugal.; I3S-Instituto de Investigação e Inovação em Saúde, University of Porto, 4200-135 Porto, Portugal., Ramalhinho AC; Health Sciences Research Centre (CICS), Faculty of Health Sciences, University of Beira Interior (UBI), Avenida Infante D. Henrique, 6200-506 Covilhã, Portugal.; Clinical Academic Centre of Beiras (CACB), Edifício UBImedical, Estrada Municipal 506, 6200-284 Covilhã, Portugal.; Cova da Beira Local Health Unit, Alameda Pêro da Covilhã, 6200-251 Covilhã, Portugal., Fonseca-Moutinho J; Health Sciences Research Centre (CICS), Faculty of Health Sciences, University of Beira Interior (UBI), Avenida Infante D. Henrique, 6200-506 Covilhã, Portugal.; Clinical Academic Centre of Beiras (CACB), Edifício UBImedical, Estrada Municipal 506, 6200-284 Covilhã, Portugal.; Cova da Beira Local Health Unit, Alameda Pêro da Covilhã, 6200-251 Covilhã, Portugal., Breitenfeld L; Health Sciences Research Centre (CICS), Faculty of Health Sciences, University of Beira Interior (UBI), Avenida Infante D. Henrique, 6200-506 Covilhã, Portugal.; Clinical Academic Centre of Beiras (CACB), Edifício UBImedical, Estrada Municipal 506, 6200-284 Covilhã, Portugal.
Jazyk: angličtina
Zdroj: Pharmaceuticals (Basel, Switzerland) [Pharmaceuticals (Basel)] 2024 Sep 12; Vol. 17 (9). Date of Electronic Publication: 2024 Sep 12.
DOI: 10.3390/ph17091201
Abstrakt: Background: Human papillomavirus (HPV), a leading cause of cervical cancer, is present in most cases of the disease and ranks as the fourth most common cancer in women globally. Among the HPV types, fourteen (HPV 16/18/31/33/35/39/45/51/52/56/58/59/66/68) are recognized as high-risk (hrHPV), each with varying levels of oncogenic potential. Detecting and genotyping these hrHPV types in cervical lesions is crucial, requiring the development of new diagnostic methods.
Methods: This study focuses on a retrospective analysis conducted on 44 women from the Cova da Beira Local Health Unit. We used the Anyplex™ II hrHPV Detection kit for hrHPV genotyping from paraffin-embedded cervical tissue samples.
Results: hrHPV types were identified in 38 out of the 44 women. Genotyping revealed HPV-16 (55.3%), HPV-18/39/56/58/59 (5.3%), HPV-31 (21.1%), HPV-35 (7.9%), HPV-51/66 (2.6%), and HPV-52 (10.5%).
Conclusions: This study demonstrates that the Anyplex™ II hrHPV Detection kit, originally designed for cervical cancer screening, is also effective for hrHPV genotyping in histological analyses. This methodology offers a simpler and more cost-effective approach for cervical cancer risk stratification. Its implementation in clinical practice could enhance the detection of hrHPV in cervical lesions, thereby contributing to more precise diagnoses and potentially more informed treatment strategies.
Databáze: MEDLINE
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