Identification of Rapeseed ( Brassica napus L.) Plant Height-Associated QTL Using BSA-seq and RNA-seq.

Autor: Xia J; College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing 400715, China., Zhan L; College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing 400715, China., Zhang J; College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing 400715, China., Song W; College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing 400715, China., Xu X; College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing 400715, China.; Academy of Agricultural Sciences, Southwest University, Beibei, Chongqing 400715, China.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2024 Sep 12; Vol. 25 (18). Date of Electronic Publication: 2024 Sep 12.
DOI: 10.3390/ijms25189875
Abstrakt: Plant height (PH) is a critical agronomic trait in Brassica napus , significantly impacting yield. Consequently, identifying genes associated with plant height is a pivotal objective in oilseed rape breeding. This study employed a combination of bulk segregant analysis sequencing (BSA-seq) and RNA sequencing (RNA-seq) for analysis. A novel quantitative trait locus (QTL), qPH_C02 , was identified between 63,989,634 and 64,945,122 bp on chromosome C02, from which eight candidate genes were screened. The Gene Ontology (GO) analysis revealed enrichment in peroxisomes, while the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated enrichment in the oxidative phosphorylation (OP) pathway. It is hypothesized that the observed differences in plant height and silique length may be attributed to the regulation of peroxidase activity in the OP pathway, which in turn alters plant energy metabolism and controls nutrient uptake. Subsequently, we will further test this hypothesis. The results of this study will contribute to our understanding of the genetic basis for differences in plant height and provide a foundation for the selection and breeding of Brassica napus varieties with desired plant shapes.
Databáze: MEDLINE
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