| Autor: |
Erel S; Department of Anesthesiology and Reanimation, Gazi University, Ankara 06560, Turkey., Küçükk A; Department of Physiology, Faculty of Medicine, Kutahya Health Sciences University, Kutahya 43020, Turkey., Dikmen K; Department of General Surgery, Faculty of Medicine, Gazi University, Ankara 06560, Turkey., Tekin E; Department of Anesthesiology and Reanimation, Gazi University, Ankara 06560, Turkey., Yığman Z; Department of Histology and Embryology, Faculty of Medicine, Gazi University, Ankara 06560, Turkey.; Neuroscience and Neurotechnology Center of Excellence NÖROM, Gazi University, Ankara 06560, Turkey., Ceyhan MŞ; Department of Histology and Embryology, Faculty of Medicine, Gazi University, Ankara 06560, Turkey., Gökgöz S; Department of Medical Biochemistry, Faculty of Medicine, Gazi University, Ankara 06560, Turkey., Bostancı H; Department of General Surgery, Faculty of Medicine, Gazi University, Ankara 06560, Turkey., Arslan M; Department of Anesthesiology and Reanimation, Gazi University, Ankara 06560, Turkey.; Life Sciences Application and Research Center, Gazi University, Ankara 06560, Turkey.; Laboratory Animal Breeding and Experimental Research Center (GUDAM), Gazi University, Ankara 06560, Turkey., Kavutcu M; Department of Medical Biochemistry, Faculty of Medicine, Gazi University, Ankara 06560, Turkey. |
| Abstrakt: |
Background and Objectives: Sepsis and its related complications are associated with high morbidity and mortality, often leading to liver damage. Ozone, a molecule with anti-inflammatory and antioxidant properties, may offer protective effects. This study aimed to evaluate the therapeutic and protective impact of ozone on liver injury in a rat model of sepsis induced by cecal ligation and perforation (CLP). Material and Methods: A total of 36 rats were randomly divided into five groups: control (Group C), ozone (Group O), cecal ligation and perforation (Group CLP), ozone + cecal ligation and perforation (Group O+CLP), and cecal ligation and perforation + ozone (Group CLP+O). In the ozone groups, 4 mL of ozone (20 µ/mL) was injected intraperitoneally. Biochemical and histopathological parameters were evaluated in liver tissue samples obtained at the end of 24 h. Results: Polymorphonuclear leukocyte and monocyte infiltration and the total injury score were significantly reduced in the ozone-treated groups compared to the CLP group ( p < 0.001). Tumor necrosis factor and interleukin 10 levels in the rat liver tissue were significantly reduced in the O+CLP and CLP+O groups compared to the CLP group, with the O+CLP group showing a more substantial decrease than the CLP+O group ( p < 0.001). Thiobarbituric acid reactive substances and glutathione s-transferase levels were significantly lower in the ozone-treated groups compared to the CLP group ( p < 0.001). Catalase activity was significantly elevated in the O+CLP group compared to the CLP group ( p < 0.001). Serum aspartate transaminase, alanine transaminase, gamma-glutamyl transferase, and total bilirubin were significantly increased in the CLP group and decreased in the ozone-treated groups ( p < 0.001, p < 0.001, p = 0.01, p < 0.001 respectively). Conclusions: Administering ozone to rats one hour before the CLP significantly mitigated liver damage, showing a more pronounced effect compared to administering ozone one hour after CLP. The results indicate that ozone could serve a protective function in managing sepsis-induced liver damage. |
