Evaluation of the Therapeutic Potential of Traditionally-Used Natural Plant Extracts to Inhibit Proliferation of a HeLa Cell Cancer Line and Replication of Human Respiratory Syncytial Virus (hRSV).

Autor:	Brill EN; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Link NG; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Jackson MR; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Alvi AF; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Moehlenkamp JN; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Beard MB; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Simons AR; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Carson LC; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Li R; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Judd BT; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Brasseale MN; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Berkman EP; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Park RK; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Cordova-Hernandez S; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Hoff RY; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Yager CE; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Modelski MC; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Nenadovich M; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Sisodia D; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Reames CJ; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Geranios AG; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Berthrong ST; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Wilson AM; Department of Chemistry and Biochemistry, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Tietje AH; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA., Stobart CC; Department of Biological Sciences, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA.; Interdisciplinary Program in Public Health, Butler University, 4600 Sunset Ave., Indianapolis, IN 46208, USA.
Jazyk:	angličtina
Zdroj:	Biology [Biology (Basel)] 2024 Sep 05; Vol. 13 (9). Date of Electronic Publication: 2024 Sep 05.
DOI:	10.3390/biology13090696
Abstrakt:	Traditional approaches employing natural plant products to treat a wide array of ailments have been documented and described for thousands of years. However, there remains limited scientific study of the therapeutic potential or effectiveness of ethnobotanical applications. Increases in the incidence of cancer and emerging infectious diseases demonstrate a growing need for advances in the development of therapeutic options. In this study, we evaluate the therapeutic potential of aqueous extracts prepared from four plants, purple aster (Symphyotrichum novae-angliae (L.) Nemsom ), common sage ( Salvia lyrata (L.)), northern spicebush ( Lindera benzoin (L.) Blume ) , and lamb's ear ( Stachys byzantina (K.) Koch )) traditionally used in Native American medicine in Indiana, USA. Using a combination of cytotoxicity assays, immunofluorescence microscopy, and antiviral assays, we found that sage and spicebush extracts exhibit cytotoxic and antiproliferative effects on HeLa cell proliferation and that sage, spicebush, and aster extracts were capable of significantly inhibiting human respiratory syncytial virus (hRSV), a major respiratory pathogen of infants and the elderly. Chemical analysis of the four extracts identified four major compounds which were subsequently evaluated to identify the responsible constituents in the extracts. While none of the identified compounds were shown to induce significant impacts on HeLa cell proliferation, two of the compounds, (1S)-(-)-Borneol and 5-(hydroxymethyl)-furfural, identified in sage and spicebush, respectively, were shown to have antiviral activities. Our data suggest that several of the extracts tested exhibited either anti-proliferative or antiviral activity supporting future further analysis.
Databáze:	MEDLINE
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