| Autor: |
Pérez-Jiménez GM; Laboratorio de Fisiología en Recursos Acuáticos (LAFIRA), División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa-Cárdenas Km. 0.5, Villahermosa 86039, Tabasco, Mexico., Alvarez-Villagomez CS; Laboratorio de Fisiología en Recursos Acuáticos (LAFIRA), División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa-Cárdenas Km. 0.5, Villahermosa 86039, Tabasco, Mexico., Martínez-Porchas M; Centro de Investigación en Alimentación y Desarrollo, A.C. Biología de Organismos Acuáticos, Hermosillo 83304, Sonora, Mexico., Garibay-Valdez E; Centro de Investigación en Alimentación y Desarrollo, A.C. Biología de Organismos Acuáticos, Hermosillo 83304, Sonora, Mexico., Sepúlveda-Quiroz CA; Laboratorio de Fisiología en Recursos Acuáticos (LAFIRA), División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa-Cárdenas Km. 0.5, Villahermosa 86039, Tabasco, Mexico.; Instituto Tecnológico de Villahermosa, Tecnológico Nacional de México, Carretera Villahermosa-Frontera, Km. 3.5, Ciudad Industrial, Villahermosa 86010, Tabasco, Mexico., Méndez-Marín O; Laboratorio de Fisiología en Recursos Acuáticos (LAFIRA), División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa-Cárdenas Km. 0.5, Villahermosa 86039, Tabasco, Mexico., Martínez-García R; Laboratorio de Fisiología en Recursos Acuáticos (LAFIRA), División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa-Cárdenas Km. 0.5, Villahermosa 86039, Tabasco, Mexico., Jesús-Contreras R; Laboratorio de Fisiología en Recursos Acuáticos (LAFIRA), División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa-Cárdenas Km. 0.5, Villahermosa 86039, Tabasco, Mexico., Alvarez-González CA; Laboratorio de Fisiología en Recursos Acuáticos (LAFIRA), División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa-Cárdenas Km. 0.5, Villahermosa 86039, Tabasco, Mexico., De la Rosa-García SDC; Laboratorio de Microbiología Aplicada (LABMIA), División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa-Cárdenas Km. 0.5, Villahermosa 86039, Tabasco, Mexico. |
| Abstrakt: |
Probiotics in aquaculture hold promise for enhancing fish health and growth. Due to their increased specificity and affinity for their host, indigenous probiotics may offer isolated and potentially amplified benefits. This study investigated the effects of Lactococcus lactis PH3-05, previously isolated from adults of tropical gar ( Atractosteus tropicus ), on the growth, survival, digestive enzyme activity, intestinal morphology, expression of barrier and immune genes, and intestinal microbiota composition in the larvae of tropical gar. Larvae were fed with live L. lactis PH3-05 concentrations of 10 4 , 10 6 , and 10 8 CFU/g for 15 days alongside a control diet without probiotics. Higher concentrations of L. lactis PH3-05 (10 6 and 10 8 CFU/g) positively influenced larval growth, increasing hepatocyte area and enterocyte height. The 10 6 CFU/g dose significantly enhanced survival (46%) and digestive enzyme activity. Notably, the 10 8 CFU/g dose stimulated increased expression of muc-2 and il-10 genes, suggesting enhanced mucosal barrier function and anti-inflammatory response. Although L. lactis PH3-05 did not significantly change the diversity, structure, or Phylum level composition of intestinal microbiota, which was constituted by Proteobacteria, Bacteroidota, Chloroflexi, and Firmicutes, an increase in Lactobacillus abundance was observed in fish fed with 10 6 CFU/g, suggesting enhanced probiotic colonization. These results demonstrate that administering L. lactis PH3-05 at 10 6 CFU/g promotes growth, survival, and digestive health in A. tropicus larvae, establishing it as a promising indigenous probiotic candidate for aquaculture applications. |
