| Autor: |
Godínez-Santillán RI; Center for Advanced Biomedical Research, School of Medicine, Autonomous University of Queretaro, Campus Aeropuerto Carretera a Chichimequillas S/N, Ejido Bolaños, Querétaro 76140, Querétaro, Mexico., Kuri-García A; Department of Cell and Molecular Biology, School of Natural Sciences, Universidad Autónoma de Querétaro, Querétaro 76230, Querétaro, Mexico., Ramírez-Pérez IF; School of Chemistry, Universidad Autónoma de Querétaro, Cerro de las Campanas, Querétaro 76076, Querétaro, Mexico., Herrera-Hernández MG; Unidad de Biotecnología, Campo Experimental Bajío, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Celaya 38110, Guanajuato, Mexico., Ahumada-Solórzano SM; Investigación Interdisciplinaria en Biomedicina, School of Natural Sciences, Universidad Autónoma de Querétaro, Querétaro 76230, Querétaro, Mexico., Guzmán-Maldonado SH; Unidad de Biotecnología, Campo Experimental Bajío, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Celaya 38110, Guanajuato, Mexico., Vergara-Castañeda HA; Center for Advanced Biomedical Research, School of Medicine, Autonomous University of Queretaro, Campus Aeropuerto Carretera a Chichimequillas S/N, Ejido Bolaños, Querétaro 76140, Querétaro, Mexico. |
| Abstrakt: |
This research identified the bioactive compounds and antioxidant capacity of the extractable (EP) and non-extractable (NEP) polyphenol fractions of berrycactus (BC). Additionally, the effects of BC and its residue (BCR) on preventing AOM/DSS-induced early colon carcinogenesis were evaluated in vivo. Male Sprague Dawley rats were randomly assigned to six groups (n = 12/group): healthy control (C), AOM/DSS, BC, BCR, BC+AOM/DSS, and BCR+AOM/DSS. NEP was obtained through acid hydrolysis using H 2 SO 4 and HCl (1 M or 4 M). The HCl-NEP fraction exhibited the highest total phenolic and flavonoid content, while condensed tannins were more abundant in the H 2 SO 4 -NEP fraction. A total of 33 polyphenols were identified by UPLC-QTOF-MSE in both EP and NEP, some of which were novel to BC. Both NEP hydrolysates demonstrated significant total antioxidant capacity (TEAC), with HCl-NEP exhibiting the highest ORAC values. The BC+AOM/DSS and BCR+AOM/DSS groups exhibited fewer aberrant crypt foci ( p < 0.05), reduced colonic epithelial injury, and presented lower fecal β-glucuronidase activity, when compared to AOM/DSS group. No differences in butyric acid concentrations were observed between groups. This study presents novel bioactive compounds in EP and NEP from BC that contribute to chemopreventive effects in early colon carcinogenesis, while reducing fecal β-glucuronidase activity and preserving colonic mucosal integrity. |
