Protective role of hesperetin in Drosophila melanogaster model of ferrous sulphate-induced toxicity.

Autor: Asejeje FO; Department of Chemical Sciences, Faculty of Natural Sciences, Ajayi Crowther University, Oyo, Oyo State, Nigeria., Asejeje GI; Drosophila Research and Training Centre, A2 Ajao Dental Street, Salami Somade Estate, Off Iyaniwura Bus Stop, Basorun, Ibadan, Oyo State, Nigeria. asejejeg@yahoo.co.uk.; Department of Chemistry, University of Ibadan, Ibadan, Oyo State, Nigeria. asejejeg@yahoo.co.uk., Ogunro OB; Department of Biological Sciences, KolaDaisi University, Ibadan, Oyo State, 2001213, Nigeria., Adedara AO; Drosophila Research and Training Centre, A2 Ajao Dental Street, Salami Somade Estate, Off Iyaniwura Bus Stop, Basorun, Ibadan, Oyo State, Nigeria., Abolaji AO; Molecular Drug Metabolism and Toxicology Unit, Department of Biochemistry, University of Ibadan, Ibadan, Oyo State, Nigeria. amos_abolaji@yahoo.com.; Drosophila Laboratory, Drug Metabolism and Toxicology Unit, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria. amos_abolaji@yahoo.com.
Jazyk: angličtina
Zdroj: BMC pharmacology & toxicology [BMC Pharmacol Toxicol] 2024 Sep 27; Vol. 25 (1), pp. 70. Date of Electronic Publication: 2024 Sep 27.
DOI: 10.1186/s40360-024-00792-0
Abstrakt: The toxicological hazard of iron-containing products is a public health concern that inspires research in identifying and developing readily available, inexpensive antidotes. Natural products, like plant-sourced antioxidants, can be of great value in this regard. Hesperetin a flavonoid abundantly present in citrus fruits is known to possess a diverse pharmacological and antioxidant attribute. The present study investigated the alleviation of detrimental effects of ferrous sulphate (FeSO 4 ) by hesperetin in Drosophila melanogaster. Flies were exposed to FeSO 4 (10 µM) alone or supplemented with hesperetin (50 or 100 µM) via diet for 7 consecutive days. Antioxidant enzyme activities, non-enzymatic antioxidant levels, acetylcholinesterase activity and oxidative stress markers were then measured. Hesperetin supplementation significantly (p < 0.05) attenuated FeSO 4 -induced oxidative stress by enhancement of enzymic antioxidants (catalase and glutathione-S-transferases) activities, preservation of non-enzymic antioxidants (total thiols and non-protein thiols), and reduction of other markers of oxidative stress (hydrogen peroxide, protein carbonyl and lipid peroxidation) in D. melanogaster. In addition, hesperetin supplementation decreased nitric oxide levels and enhanced acetylcholinesterase activity. Furthermore, hesperetin supplementation improved FeSO 4 -induced locomotor deficit, while there was no significant difference in cell viability (mitochondrial metabolic rate) in the treatment groups. This study suggests that hesperetin might be a promising functional agent in preventing iron toxicity and similar metal-induced impairments.
(© 2024. The Author(s).)
Databáze: MEDLINE
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