Evolutionary origin and population diversity of a cryptic hybrid pathogen.

Autor: Steenwyk JL; Howards Hughes Medical Institute and the Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, USA.; Vanderbilt University, Department of Biological Sciences, VU Station B #35-1634, Nashville, USA.; Evolutionary Studies Initiative, Vanderbilt University, Nashville, USA., Knowles S; Department of Chemistry & Biochemistry, University of North Carolina at Greensboro, Greensboro, USA., Bastos RW; Faculdade de Ciencias Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.; Department of Microbiology and Parasitology, Bioscience Center, Federal University of Rio Grande do Norte, Natal-RN, Brazil., Balamurugan C; Vanderbilt University, Department of Biological Sciences, VU Station B #35-1634, Nashville, USA.; Evolutionary Studies Initiative, Vanderbilt University, Nashville, USA., Rinker D; Vanderbilt University, Department of Biological Sciences, VU Station B #35-1634, Nashville, USA.; Evolutionary Studies Initiative, Vanderbilt University, Nashville, USA., Mead ME; Vanderbilt University, Department of Biological Sciences, VU Station B #35-1634, Nashville, USA.; Evolutionary Studies Initiative, Vanderbilt University, Nashville, USA.; Ginkgo Bioworks, 27 Drydock Avenue, 8th Floor, Boston, USA., Roberts CD; Department of Chemistry & Biochemistry, University of North Carolina at Greensboro, Greensboro, USA., Raja HA; Department of Chemistry & Biochemistry, University of North Carolina at Greensboro, Greensboro, USA., Li Y; Institute of Marine Science and Technology, Shandong University, 72 Binhai Road, Qingdao, China., Colabardini AC; Faculdade de Ciencias Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil., de Castro PA; Faculdade de Ciencias Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil., Dos Reis TF; Faculdade de Ciencias Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil., Gumilang A; Vanderbilt University, Department of Biological Sciences, VU Station B #35-1634, Nashville, USA.; Evolutionary Studies Initiative, Vanderbilt University, Nashville, USA., Almagro-Molto M; Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Faculty of Medicine, Ludwig Maximilian University, Munich, Germany., Alanio A; Institut Pasteur, Paris Cité University, National Reference Center for Invasives Mycoses and Antifungals, Translational Mycology Research Group, Mycology Department, Paris, France.; Laboratoire de parasitologie-mycologie, AP-HP, Hôpital Saint-Louis, Paris, France., Garcia-Hermoso D; Institut Pasteur, Paris Cité University, National Reference Center for Invasives Mycoses and Antifungals, Translational Mycology Research Group, Mycology Department, Paris, France., Delbaje E; Faculdade de Ciencias Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil., Pontes L; Faculdade de Ciencias Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil., Pinzan CF; Faculdade de Ciencias Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil., Schreiber AZ; School of Medical Sciences, State University of Campinas, Campinas, Brazil., Canóvas D; Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Seville, Spain.; Clinical Microbiology Unit. Synlab Laboratory at Viamed Sta. Ángela de la Cruz Hospital, Seville, Spain., Sanchez Luperini R; Faculdade de Ciencias Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil., Lagrou K; Department of Microbiology, Immunology and Transplantation, Katholieke Universiteit Leuven, Leuven, Belgium.; Department of Laboratory Medicine and National Reference Centre for Mycosis, University Hospitals Leuven, Leuven, Belgium., Torrado E; Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4715-495 Braga, Portugal; ICVS/3B's-PT Government Associate Laboratory, Braga, Portugal., Rodrigues F; Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4715-495 Braga, Portugal; ICVS/3B's-PT Government Associate Laboratory, Braga, Portugal., Oberlies NH; Department of Chemistry & Biochemistry, University of North Carolina at Greensboro, Greensboro, USA., Zhou X; Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, China., Goldman GH; Faculdade de Ciencias Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil. ggoldman@usp.br.; National Institute of Science and Technology in Human Pathogenic, Fungi, Brazil. ggoldman@usp.br., Rokas A; Vanderbilt University, Department of Biological Sciences, VU Station B #35-1634, Nashville, USA. antonis.rokas@vanderbilt.edu.; Evolutionary Studies Initiative, Vanderbilt University, Nashville, USA. antonis.rokas@vanderbilt.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Sep 28; Vol. 15 (1), pp. 8412. Date of Electronic Publication: 2024 Sep 28.
DOI: 10.1038/s41467-024-52639-1
Abstrakt: Cryptic fungal pathogens pose disease management challenges due to their morphological resemblance to known pathogens. Here, we investigated the genomes and phenotypes of 53 globally distributed isolates of Aspergillus section Nidulantes fungi and found 30 clinical isolates-including four isolated from COVID-19 patients-were A. latus, a cryptic pathogen that originated via allodiploid hybridization. Notably, all A. latus isolates were misidentified. A. latus hybrids likely originated via a single hybridization event during the Miocene and harbor substantial genetic diversity. Transcriptome profiling of a clinical isolate revealed that both parental subgenomes are actively expressed and respond to environmental stimuli. Characterizing infection-relevant traits-such as drug resistance and growth under oxidative stress-revealed distinct phenotypic profiles among A. latus hybrids compared to parental and closely related species. Moreover, we identified four features that could aid A. latus taxonomic identification. Together, these findings deepen our understanding of the origin of cryptic pathogens.
(© 2024. The Author(s).)
Databáze: MEDLINE
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