Uridylation regulates mRNA decay directionality in fission yeast.

Autor: Grochowski M; Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland., Lipińska-Zubrycka L; Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland., Townsend S; Department of Biochemistry, Charité - Universitätsmedizin Berlin, Berlin, Germany.; Molecular Biology of Metabolism Laboratory, Francis Crick Institute, London, United Kingdom., Golisz-Mocydlarz A; Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland., Zakrzewska-Płaczek M; Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland., Brzyżek G; Laboratory of Seeds Molecular Biology, Institute of Biochemistry and Biophysics, PAS, Warsaw, Poland., Jurković B; Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland., Świeżewski S; Laboratory of Seeds Molecular Biology, Institute of Biochemistry and Biophysics, PAS, Warsaw, Poland., Ralser M; Department of Biochemistry, Charité - Universitätsmedizin Berlin, Berlin, Germany.; Molecular Biology of Metabolism Laboratory, Francis Crick Institute, London, United Kingdom., Małecki M; Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland. m.malecki@igib.uw.edu.pl.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Sep 27; Vol. 15 (1), pp. 8359. Date of Electronic Publication: 2024 Sep 27.
DOI: 10.1038/s41467-024-50824-w
Abstrakt: Cytoplasmic mRNA decay is effected by exonucleolytic degradation in either the 5' to 3' or 3' to 5' direction. Pervasive terminal uridylation is implicated in mRNA degradation, however, its functional relevance for bulk mRNA turnover remains poorly understood. In this study, we employ genome-wide 3'-RACE (gw3'-RACE) in the model system fission yeast to elucidate the role of uridylation in mRNA turnover. We observe widespread uridylation of shortened poly(A) tails, promoting efficient 5' to 3' mRNA decay and ensuring timely and controlled mRNA degradation. Inhibition of this uridylation process leads to excessive deadenylation and enhanced 3' to 5' mRNA decay accompanied by oligouridylation. Strikingly we found that uridylation of poly(A) tails and oligouridylation of non-polyadenylated substrates are catalysed by different terminal uridyltransferases Cid1 and Cid16 respectively. Our study sheds new light on the intricate regulatory mechanisms underlying bulk mRNA turnover, demonstrating the role of uridylation in modulating mRNA decay pathways.
(© 2024. The Author(s).)
Databáze: MEDLINE
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