An alternative method for comparing hemorrhagic activity of snake venoms following one of the 3R's principles.

Autor: Tasima LJ; Laboratory of Herpetology, Butantan Institute, São Paulo, 05503-900, SP, Brazil; Interunidades em Biotecnologia, Instituto de Ciências Biomédicas-Instituto de Pesquisas Tecnológicas-Instituto Butantan, Universidade de São Paulo, São Paulo, 05508-900, SP, Brazil., Kavazoi VK; Laboratory of Herpetology, Butantan Institute, São Paulo, 05503-900, SP, Brazil; Interunidades em Biotecnologia, Instituto de Ciências Biomédicas-Instituto de Pesquisas Tecnológicas-Instituto Butantan, Universidade de São Paulo, São Paulo, 05508-900, SP, Brazil., Lima EOV; Laboratory of Herpetology, Butantan Institute, São Paulo, 05503-900, SP, Brazil; Interunidades em Biotecnologia, Instituto de Ciências Biomédicas-Instituto de Pesquisas Tecnológicas-Instituto Butantan, Universidade de São Paulo, São Paulo, 05508-900, SP, Brazil., Grego KF; Laboratory of Herpetology, Butantan Institute, São Paulo, 05503-900, SP, Brazil., Tanaka-Azevedo AM; Laboratory of Herpetology, Butantan Institute, São Paulo, 05503-900, SP, Brazil; Interunidades em Biotecnologia, Instituto de Ciências Biomédicas-Instituto de Pesquisas Tecnológicas-Instituto Butantan, Universidade de São Paulo, São Paulo, 05508-900, SP, Brazil. Electronic address: anita.azevedo@butantan.gov.br.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2024 Nov 06; Vol. 250, pp. 108111. Date of Electronic Publication: 2024 Sep 26.
DOI: 10.1016/j.toxicon.2024.108111
Abstrakt: Venom-induced hemorrhage analysis usually is performed by Minimum Hemorrhagic Dose (MHD), however a similar method can be used to compare venoms with fewer laboratory animals. Our work compared the MHD of five different venoms, with the size of hemorrhagic spot, finding good correlations in the results. Considering the 3Rs principle, we propose the use of the hemorrhagic spot method to compare hemorrhagic activity of snake venoms, rather than using the MHD method, since the first one needs 5 times less animals than the other.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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