Photodisinfection of Alphaherpesvirus 1 in bovine semen.

Autor: Dos Anjos Oliveira TM; Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Goiânia, GO, Brazil., Teles AV; Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Goiânia, GO, Brazil., Gambarini ML; Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Goiânia, GO, Brazil., de Oliveira Ribeiro K; Escola de Agronomia, Universidade Federal de Goiás, Goiânia, GO, Brazil., Ducas ESA; Instituto de Química, Universidade Federal de Goiás, Goiânia, GO, Brazil., Dos Santos KJG; Centro de Biotecnologia em Reprodução Animal (BIOTEC), Universidade Estadual de Goiás, São Luís de Montes Belos, GO, Brazil., Monteiro CJP; LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal., de Paula Silveira Lacerda E; Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil., Franchi LP; Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil., Gonçalves PJ; Instituto de Química, Universidade Federal de Goiás, Goiânia, GO, Brazil; Instituto de Física, Universidade Federal de Goiás, Goiânia, GO, Brazil; Centro de Excelência em Hidrogênio e Tecnologias Energéticas Sustentáveis (CEHTES), Goiânia, GO, Brazil. Electronic address: pablo@ufg.br., de Souza GRL; Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Goiânia, GO, Brazil; Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil. Electronic address: guilherme_lino@ufg.br.
Jazyk: angličtina
Zdroj: Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2024 Nov; Vol. 260, pp. 113036. Date of Electronic Publication: 2024 Sep 24.
DOI: 10.1016/j.jphotobiol.2024.113036
Abstrakt: Reproductive biotechnologies are widely consolidated as a methodology in cattle breeding and have an important impact on the genetic improvement of cattle herds. Semen is an important source of dissemination of pathogenic microorganisms during reproductive procedures. To ensure the sanitary quality of the semen, it is essential to consider the presence of various microorganisms including viruses. One of the main viral agents of reproductive interest is Bovine Alphaherpesvirus 1 (BoHV-1), the etiological agent responsible for bovine rhinotracheitis and vulvovaginitis and frequently associated with reproductive efficiency of matrices and bulls. In artificial insemination centers, semen treatment is generally based only on the use of antibiotics, ignoring the possibility of inactivating other non-bacterial infectious agents. In this context, photodisinfection emerges as a promising alternative to inactivate a wide range of microorganisms, offering a complementary or substitution approach to those conventional semen treatment methods. In this work, we evaluated the use of four halogenated sulfonated porphyrins as potential photosensitizers (PSs) for photodynamic inactivation of Bovine Alphaherpesvirus I (BoHV-1) for bovine semen disinfection. The PSs were synthesized and photophysical parameters, such as UV-Vis absorption spectra and singlet oxygen quantum yield (Φ Δ ) were presented. Photoinactivation of BoHV-1 was first shown in cell culture and then confirmed in artificially infected bovine semen and then the phototoxicity of PSs against spermatozoa was evaluated. All PSs were effective in BoHV-1 inactivation; however, the photosensitizer containing two chlorine atoms, showed to be more efficient due to the shorter time required for complete viral inactivation. The slight alterations in sperm kinetics were observed, but remained within those acceptable by regulatory agencies for animal reproduction. Although the methodology used in this work only included bovine semen, we emphasize that the proposed photodisinfection methodology can be adapted and applied to a wide range of biological materials and microorganisms of animal or human interest.
Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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