The Contrasting Effects of Bothrops lanceolatus and Bothrops atrox Venom on Procoagulant Activity and Thrombus Stability under Blood Flow Conditions.

Autor: Radouani F; Cardiovascular Research Team (UR5_3 PC2E), University of the French West Indies (Université des Antilles), 97200 Fort-de-France, France.; Department of Research, University Hospital of Martinique (CHU Martinique), 97261 Fort-de-France, France., Jalta P; Cardiovascular Research Team (UR5_3 PC2E), University of the French West Indies (Université des Antilles), 97200 Fort-de-France, France.; Department of Research, University Hospital of Martinique (CHU Martinique), 97261 Fort-de-France, France., Rapon C; Cardiovascular Research Team (UR5_3 PC2E), University of the French West Indies (Université des Antilles), 97200 Fort-de-France, France., Lezin C; Cardiovascular Research Team (UR5_3 PC2E), University of the French West Indies (Université des Antilles), 97200 Fort-de-France, France.; Department of Biology, Faculté des Sciences Exactes et Naturelles (SEN), Campus Fouillole, 97157 Pointe-à-Pitre, France., Branford C; Cardiovascular Research Team (UR5_3 PC2E), University of the French West Indies (Université des Antilles), 97200 Fort-de-France, France., Florentin J; Cardiovascular Research Team (UR5_3 PC2E), University of the French West Indies (Université des Antilles), 97200 Fort-de-France, France.; Department of Research, University Hospital of Martinique (CHU Martinique), 97261 Fort-de-France, France., Gutierrez JM; Clodomiro Picado Institute, School of Microbiology, University of Costa Rica, San José 11501, Costa Rica., Resiere D; Cardiovascular Research Team (UR5_3 PC2E), University of the French West Indies (Université des Antilles), 97200 Fort-de-France, France.; Department of Toxicology and Critical Care Medicine, University Hospital of Martinique (CHU Martinique), 97261 Fort-de-France, France., Neviere R; Cardiovascular Research Team (UR5_3 PC2E), University of the French West Indies (Université des Antilles), 97200 Fort-de-France, France.; Department of Clinical Physiology, University Hospital of Martinique (CHU Martinique), 97261 Fort-de-France, France., Pierre-Louis O; Cardiovascular Research Team (UR5_3 PC2E), University of the French West Indies (Université des Antilles), 97200 Fort-de-France, France.
Jazyk: angličtina
Zdroj: Toxins [Toxins (Basel)] 2024 Sep 18; Vol. 16 (9). Date of Electronic Publication: 2024 Sep 18.
DOI: 10.3390/toxins16090400
Abstrakt: Background: Consumption coagulopathy and hemorrhagic syndrome are the typical features of Bothrops sp. snake envenoming. In contrast, B. lanceolatus envenoming can induce thrombotic complications. Our aim was to test whether crude B. lanceolatus and B. atrox venoms would display procoagulant activity and induce thrombus formation under flow conditions.
Methods and Principal Findings: Fibrin formation in human plasma was observed for B. lanceolatus venom at 250-1000 ng/mL concentrations, which also induced clot formation in purified human fibrinogen, indicating thrombin-like activity. The degradation of fibrinogen confirmed the fibrinogenolytic activity of B. lanceolatus venom. B. lanceolatus venom displayed consistent thrombin-like and kallikrein-like activity increases in plasma conditions. The well-known procoagulant B. atrox venom activated plasmatic coagulation factors in vitro and induced firm thrombus formation under high shear rate conditions. In contrast, B. lanceolatus venom induced the formation of fragile thrombi that could not resist shear stress.
Conclusions: Our results suggest that crude B. lanceolatus venom displays amidolytic activity and can activate the coagulation cascade, leading to prothrombin activation. B. lanceolatus venom induces the formation of an unstable thrombus under flow conditions, which can be prevented by the specific monovalent antivenom Bothrofav ® .
Databáze: MEDLINE
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