Chalkbrood Disease Caused by Ascosphaera apis in Honey Bees ( Apis mellifera )-Morphological and Histological Changes in Infected Larvae.

Autor:	von Knoblauch T; LABOKLIN GmbH & Co. KG, Labor Für Klinische Diagnostik, Steubenstraße 4, 97688 Bad Kissingen, Germany., Jensen AB; Department of Plant and Environmental Sciences Section for Organismal Biology, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark., Mülling CKW; Institute of Veterinary Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 43, 04103 Leipzig, Germany., Aupperle-Lellbach H; LABOKLIN GmbH & Co. KG, Labor Für Klinische Diagnostik, Steubenstraße 4, 97688 Bad Kissingen, Germany., Genersch E; Department of Molecular Microbiology and Bee Diseases, Institute for Bee Research, Friedrich-Engels-Str. 32, 16540 Hohen Neuendorf, Germany.; Institute of Microbiology and Epizootics, Faculty of Veterinary Medicine, Freie Universität Berlin, Robert-von-Ostertag-Str. 7, 14163 Berlin, Germany.
Jazyk:	angličtina
Zdroj:	Veterinary sciences [Vet Sci] 2024 Sep 06; Vol. 11 (9). Date of Electronic Publication: 2024 Sep 06.
DOI:	10.3390/vetsci11090415
Abstrakt:	Chalkbrood is a mycological brood disease of the Western honey bee ( Apis mellifera ), caused by the fungus Ascosphaera apis . The aim of this study was the investigation of the pathology of artificially reared Apis mellifera larvae, experimentally infected with A. apis spores (1.0 × 10 3 spores/larva). Non-infected larvae served as control. Five living larvae and every dead larva were collected daily (day 1-7 p.i.). All larvae were macroscopically measured, photographed, formalin-fixed, and histologically processed (hematoxylin-eosin stain, Grocott silvering). Histological sections were digitized, and the size of the larvae was measured (mouth-after length, area) and statistically analyzed. Twenty-six larvae from the collected larvae ( n = 64; 23 dead, 3 alive) showed histological signs of infection from 3 d p.i. onwards. The dead larvae showed macroscopically white/brown deposits, indistinct segmentation, and a lack of body elongation. Infected larvae were significantly smaller than the controls on days 3 p.i. ( p < 0.05), 4 p.i. ( p < 0.001), and 6 p.i. ( p < 0.05). The early time of death, the low number of transitional stages, and the strong penetration of the larval carcass with fungal mycelium indicate a rapid and fulminant infection process, which is probably relevant for spreading the disease within the colony. Competing Interests: Authors T.v.K. and H.A.-L. were employed by the company LABOKLIN GmbH & CO. KG. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu