| Autor: |
Rahman MS; Institution of Nutrition and Functional Foods, Faculty Agricultural and Food Sciences, Laval University, Laval, QC G1V 0A6, Canada., Alam MB; Inner Beauty/Antiaging Center, Food and Bio-Industry Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea., Naznin M; Department of Chemistry, Kyungpook National University, Daegu 41566, Republic of Korea., Madina MH; Institution of Nutrition and Functional Foods, Faculty Agricultural and Food Sciences, Laval University, Laval, QC G1V 0A6, Canada., Rafiquzzaman SM; Department of Fisheries Biology and Aquatic Environment, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh. |
| Jazyk: |
angličtina |
| Zdroj: |
Marine drugs [Mar Drugs] 2024 Aug 26; Vol. 22 (9). Date of Electronic Publication: 2024 Aug 26. |
| DOI: |
10.3390/md22090383 |
| Abstrakt: |
This study aimed to assess the anti-inflammatory properties of a bioactive glutamic-alanine rich glycoprotein (GP) derived from Undaria pinnatifida on both LPS-stimulated RAW264.7 cells, peritoneal macrophages, and mouse models of carrageenan- and xylene-induced inflammation, investigating the underlying molecular mechanisms. In both in-vitro and in-vivo settings, GP was found to reduce the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) while also inhibiting the production of nitric oxide (NO) and prostaglandin E 2 (PGE 2 ) in response to lipopolysaccharide (LPS) stimulation. GP treatment significantly impeded the nuclear translocation of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway by blocking the phosphorylation of IKKα and IκBα, leading to a reduction in proinflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6). Additionally, GP effectively inhibited the activation of mitogen-activated protein kinases (MAPKs), with specific inhibitors of p38 and extra-cellular signal regulated kinase (ERK) enhancing GP's anti-inflammatory efficacy. Notably, GP administration at 10 mg/kg/day (p.o.) markedly reduced carrageenan-induced paw inflammation and xylene-induced ear edema by preventing the infiltration of inflammatory cells into targeted tissues. GP treatment also downregulated key inflammatory markers, including iNOS, COX-2, IκBα, and NF-κB, by suppressing the phosphorylation of p38 and ERK, thereby improving the inflammatory index in both carrageenan- and xylene-induced mouse models. These findings suggest that marine resources, particularly seaweeds like U. pinnatifida , could serve as valuable sources of natural anti-inflammatory proteins for the effective treatment of inflammation and related conditions. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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