| Autor: |
Bahloul B; Drug Development Laboratory LR12ES09, Faculty of Pharmacy, University of Monastir, Monastir 5000, Tunisia., Ben Bnina E; LR21AGR03-Production and Protection for a Sustainable Horticulture (2PHD), IRESA-University of Sousse, Regional Research Centre on Horticulture and Organic Agriculture, Sousse 4042, Tunisia., Dridi D; Drug Development Laboratory LR12ES09, Faculty of Pharmacy, University of Monastir, Monastir 5000, Tunisia., Bouhamed A; Drug Development Laboratory LR12ES09, Faculty of Pharmacy, University of Monastir, Monastir 5000, Tunisia., Castillo Henríquez L; CNRS, INSERM, UTCBS, Chemical and Biological Technologies for Health Laboratory, Université Paris Cité, F-75006 Paris, France., Flamini G; Dipartimento di Farmacia, Via Bonanno 6, 56126 Pisa, Italy., Vega-Baudrit JR; National Nanotechnology Laboratory (LANOTEC), National Center for High Technology (CeNAT), San José 1174-1200, Costa Rica. |
| Abstrakt: |
The aim of this study is to develop a nanoemulgel encapsulating a Tunisian Prickly Pear ( Opuntia ficus-indica L.) seed oil (PPSO) to assess, for the first time, the in vivo efficacy of this nanoformulation on wound healing. Phytocompounds of this oil have been reported in the literature as having powerful pharmacological activities. However, it remains poorly exploited due to low bioavailability. A nanoemulsion (NE) was designed by determining the required hydrophilic-lipophilic balance (HLB) and subsequently characterized. The mean droplet size was measured at 56.46 ± 1.12 nm, with a polydispersity index (PDI) of 0.23 ± 0.01 using dynamic light scattering. The zeta potential was -31.4 ± 1.4 mV, and the morphology was confirmed and assessed using transmission electron microscopy (TEM). These characteristics align with the typical properties of nanoemulsions. The gelification process resulted in the formation of a nanoemulgel from the optimum nanoemulsion. The high wound healing efficiency of the nanoemulgel was confirmed compared to that of a medicinally marketed cream. The outcomes of this research contribute valuable insights, for the first time, into the potential therapeutic applications of PPSO and its innovative pharmaceutical formulation for wound healing. |
