| Autor: |
Vishnevetskii DV; Department of Physical Chemistry, Tver State University, Building 33, Zhelyabova Str., Tver 170100, Russia.; Institute of Biomedical Chemistry, 10 Building 8, Pogodinskaya Str., Moscow 191121, Russia., Metlin FA; Department of Physical Chemistry, Tver State University, Building 33, Zhelyabova Str., Tver 170100, Russia., Andrianova YV; Department of Physical Chemistry, Tver State University, Building 33, Zhelyabova Str., Tver 170100, Russia., Polyakova EE; Department of Physical Chemistry, Tver State University, Building 33, Zhelyabova Str., Tver 170100, Russia., Ivanova AI; Department of Applied Physics, Tver State University, Building 33, Zhelyabova Str., Tver 170100, Russia., Averkin DV; Russian Metrological Institute of Technical Physics and Radio Engineering, Worker's Settlement Mendeleevo, Building 11, Moscow 141570, Russia., Mekhtiev AR; Institute of Biomedical Chemistry, 10 Building 8, Pogodinskaya Str., Moscow 191121, Russia. |
| Abstrakt: |
In this study, a novel supramolecular composite, "photogels", was synthesized by mixing of cysteine-silver sol (CSS) and methylene blue (MB). A complex of modern physico-chemical methods of analysis such as viscosimetry, UV spectroscopy, dynamic and electrophoretic light scattering, scanning electron microscopy and energy-dispersive X-ray spectroscopy showed that MB molecules are uniformly localized mainly in the space between fibers of the gel-network formed by CSS particles. Molecules of the dye also bind with the surface of CSS particles by non-covalent interactions. This fact is reflected in the appearance of a synergistic anticancer effect of gels against human squamous cell carcinoma even in the absence of light irradiation. A mild toxic influence of hydrogels was observed in normal keratinocyte cells. Photodynamic exposure significantly increased gel activity, and there remained a synergistic effect. The study of free-radical oxidation in cells has shown that gels are not only capable of generating reactive oxygen species, but also have other targets of action. Flow cytometric analysis allowed us to find out that obtained hydrogels caused cell cycle arrest both without irradiation and with light exposure. The obtained gels are of considerable interest both from the point of view of academics and applied science, for example, in the photodynamic therapy of superficial neoplasms. |
