| Autor: |
Trainito A; IRCCS Centro Neurolesi 'Bonino-Pulejo', Via Provinciale Palermo, Contrada Casazza, 98124 Messina, Italy., Muscarà C; IRCCS Centro Neurolesi 'Bonino-Pulejo', Via Provinciale Palermo, Contrada Casazza, 98124 Messina, Italy., Gugliandolo A; IRCCS Centro Neurolesi 'Bonino-Pulejo', Via Provinciale Palermo, Contrada Casazza, 98124 Messina, Italy., Chiricosta L; IRCCS Centro Neurolesi 'Bonino-Pulejo', Via Provinciale Palermo, Contrada Casazza, 98124 Messina, Italy., Salamone S; Department of Pharmaceutical Sciences, University of Eastern Piedmont, Largo Donegani 2, 28100 Novara, Italy., Pollastro F; Department of Pharmaceutical Sciences, University of Eastern Piedmont, Largo Donegani 2, 28100 Novara, Italy., Mazzon E; Department of Medical, Oral and Biotechnological Sciences, University 'G. d'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., D'Angiolini S; IRCCS Centro Neurolesi 'Bonino-Pulejo', Via Provinciale Palermo, Contrada Casazza, 98124 Messina, Italy. |
| Abstrakt: |
Neurological disorders such as Alzheimer's, Parkinson's, amyotrophic lateral sclerosis, and schizophrenia are associated with altered neuronal excitability, resulting from dysfunctions in the molecular architecture and physiological regulation of ion channels and synaptic transmission. Ion channels and synapses are regarded as suitable therapeutic targets in modern pharmacology. Cannabinoids have received great attention as an original therapeutic approach for their effects on human health due to their ability to modulate the neurotransmitter release through interaction with the endocannabinoid system. In our study, we explored the effect of cannabinol (CBN) through next-generation sequencing analysis of NSC-34 cell physiology. Our findings revealed that CBN strongly influences the ontologies related to ion channels and synapse activity at all doses tested. Specifically, the genes coding for calcium and potassium voltage-gated channel subunits, and the glutamatergic and GABAergic receptors ( Cacna1b , Cacna1h , Cacng8 , Kcnc3 , Kcnd1 , Kcnd2 , Kcnj4 , Grik5 , Grik1 , Slc17a7 , Gabra5 ), were up-regulated. Conversely, the genes involved into serotoninergic and cholinergic pathways ( Htr3a , Htr3b , Htr1b , Chrna3 , Chrnb2 , Chrnb4 ), were down-regulated. These findings highlight the influence of CBN in the expression of genes involved into ion influx and synaptic transmission. |
