3D Modeling: Insights into the Metabolic Reprogramming of Cholangiocarcinoma Cells.

Autor: Ciufolini G; Department of Chemical Science and Technology, University of Rome 'Tor Vergata', 00133 Rome, Italy., Zampieri S; Department of Chemical Science and Technology, University of Rome 'Tor Vergata', 00133 Rome, Italy., Cesaroni S; Department of Chemical Science and Technology, University of Rome 'Tor Vergata', 00133 Rome, Italy., Pasquale V; Department of Biotechnology and Biosciences, University of Milan-Bicocca, 20126 Milan, Italy.; SYSBIO-ISBE-IT-Candidate National Node of Italy for ISBE, Research Infrastructure for Systems Biology Europe, 20126 Milan, Italy., Bonanomi M; SYSBIO-ISBE-IT-Candidate National Node of Italy for ISBE, Research Infrastructure for Systems Biology Europe, 20126 Milan, Italy.; Institute of Bioimaging and Complex Biological Systems (IBSBC), 20054 Segrate, Italy., Gaglio D; SYSBIO-ISBE-IT-Candidate National Node of Italy for ISBE, Research Infrastructure for Systems Biology Europe, 20126 Milan, Italy.; Institute of Bioimaging and Complex Biological Systems (IBSBC), 20054 Segrate, Italy., Sacco E; Department of Biotechnology and Biosciences, University of Milan-Bicocca, 20126 Milan, Italy.; SYSBIO-ISBE-IT-Candidate National Node of Italy for ISBE, Research Infrastructure for Systems Biology Europe, 20126 Milan, Italy., Vanoni M; Department of Biotechnology and Biosciences, University of Milan-Bicocca, 20126 Milan, Italy.; SYSBIO-ISBE-IT-Candidate National Node of Italy for ISBE, Research Infrastructure for Systems Biology Europe, 20126 Milan, Italy., Pastore M; Department of Experimental and Clinical Medicine, University of Florence, 50121 Florence, Italy., Marra F; Department of Experimental and Clinical Medicine, University of Florence, 50121 Florence, Italy., Cicero DO; Department of Chemical Science and Technology, University of Rome 'Tor Vergata', 00133 Rome, Italy., Raggi C; Department of Experimental and Clinical Medicine, University of Florence, 50121 Florence, Italy., Petrella G; Department of Chemical Science and Technology, University of Rome 'Tor Vergata', 00133 Rome, Italy.
Jazyk: angličtina
Zdroj: Cells [Cells] 2024 Sep 13; Vol. 13 (18). Date of Electronic Publication: 2024 Sep 13.
DOI: 10.3390/cells13181536
Abstrakt: Developing accurate in vitro models that replicate the in vivo tumor environment is essential for advancing cancer research and therapeutic development. Traditional 2D cell cultures often fail to capture the complex structural and functional heterogeneity of tumors, limiting the translational relevance of findings. In contrast, 3D culture systems, such as spheroids, provide a more physiologically relevant context by replicating key aspects of the tumor microenvironment. This study aimed to compare the metabolism of three intrahepatic cholangiocarcinoma cell lines in 2D and 3D cultures to identify metabolic shifts associated with spheroid formation. Cells were cultured in 2D on adhesion plates and in 3D using ultra-low attachment plates. Metabolic exchange rates were measured using NMR, and intracellular metabolites were analyzed using LC-MS. Significant metabolic differences were observed between 2D and 3D cultures, with notable changes in central carbon and glutathione metabolism in 3D spheroids. The results suggest that 3D cultures, which more closely mimic the in vivo environment, may offer a more accurate platform for cancer research and drug testing.
Databáze: MEDLINE
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