| Autor: |
Fiorelli E; Anatomic Pathology Section, Department of Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy., Scioli MG; Anatomic Pathology Section, Department of Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy., Terriaca S; Anatomic Pathology Section, Department of Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy.; Plastic Surgery Unit, Department of Surgery 'Pietro Valdoni', Sapienza University of Rome, 00133 Rome, Italy., Ul Haq A; Department of Industrial Engineering, University of Rome Tor Vergata, 00133 Rome, Italy.; Interdepartmental Research Centre for Regenerative Medicine (CIMER), University of Rome Tor Vergata, 00133 Rome, Italy., Storti G; Department of Plastic Surgery, University of Rome Tor Vergata, 00133 Rome, Italy., Madaghiele M; Department of Experimental Medicine, University of Salento, 73100 Lecce, Italy., Palumbo V; Department of Experimental Medicine, University of Rome Tor Vergata, 00133 Rome, Italy., Pashaj E; Department of Surgical Sciences, Catholic University Our Lady of Good Counsel, 1005 Tirana, Albania., De Matteis F; Department of Industrial Engineering, University of Rome Tor Vergata, 00133 Rome, Italy.; Interdepartmental Research Centre for Regenerative Medicine (CIMER), University of Rome Tor Vergata, 00133 Rome, Italy., Ribuffo D; Plastic Surgery Unit, Department of Surgery 'Pietro Valdoni', Sapienza University of Rome, 00133 Rome, Italy., Cervelli V; Department of Plastic Surgery, University of Rome Tor Vergata, 00133 Rome, Italy., Orlandi A; Anatomic Pathology Section, Department of Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy.; Department of Biomedical Sciences, Catholic University Our Lady of Good Counsel, 1005 Tirana, Albania. |
| Abstrakt: |
Osteochondral lesions may be due to trauma or congenital conditions. In both cases, therapy is limited because of the difficulty of tissue repair. Tissue engineering is a promising approach that relies on designed scaffolds with variable mechanical attributes to favor cell attachment and differentiation. Human adipose-derived stem cells (hASCs) are a very promising cell source in regenerative medicine with osteochondrogenic potential. Based on the assumption that stiffness influences cell commitment, we investigated three different scaffolds: a semisynthetic animal-derived GelMA hydrogel, a combined scaffold made of rigid PEGDA coated with a thin GelMA layer and a decellularized plant-based scaffold. We investigated the role of different biomechanical stimulations in the scaffold-induced osteochondral differentiation of hASCs. We demonstrated that all scaffolds support cell viability and spontaneous osteochondral differentiation without any exogenous factors. In particular, we observed mainly osteogenic commitment in higher stiffness microenvironments, as in the plant-based one, whereas in a dense and softer matrix, such as in GelMA hydrogel or GelMA-coated-PEGDA scaffold, chondrogenesis prevailed. We can induce a specific cell commitment by combining hASCs and scaffolds with particular mechanical attributes. However, in vivo studies are needed to fully elucidate the regenerative process and to eventually suggest it as a potential approach for regenerative medicine. |
