| Autor: |
Simó-Cabrera L; Biochemistry, Molecular Biology, Edaphology and Agricultural Chemistry Department, Faculty of Science, University of Alicante, Carretera San Vicente del Raspeig s/n-03690 San Vicente del Raspeig, E-03690 Alicante, Spain.; Multidisciplinary Institute for Environmental Studies 'Ramón Margalef', University of Alicante, Ap. 99, E-03080 Alicante, Spain., García-Chumillas S; Technological Centre of Footwear and Plastic of the Region of Murcia (CETEC) Avda, Europa 4-5, E-30840 Alhama de Murcia, Spain.; Cetec Biotechnology, Avda, Europa 4-5, E-30840 Alhama de Murcia, Spain., Benitez-Benitez SJ; Technological Centre of Footwear and Plastic of the Region of Murcia (CETEC) Avda, Europa 4-5, E-30840 Alhama de Murcia, Spain., Cánovas V; Cetec Biotechnology, Avda, Europa 4-5, E-30840 Alhama de Murcia, Spain., Monzó F; Technological Centre of Footwear and Plastic of the Region of Murcia (CETEC) Avda, Europa 4-5, E-30840 Alhama de Murcia, Spain., Pire C; Biochemistry, Molecular Biology, Edaphology and Agricultural Chemistry Department, Faculty of Science, University of Alicante, Carretera San Vicente del Raspeig s/n-03690 San Vicente del Raspeig, E-03690 Alicante, Spain.; Multidisciplinary Institute for Environmental Studies 'Ramón Margalef', University of Alicante, Ap. 99, E-03080 Alicante, Spain., Martínez-Espinosa RM; Biochemistry, Molecular Biology, Edaphology and Agricultural Chemistry Department, Faculty of Science, University of Alicante, Carretera San Vicente del Raspeig s/n-03690 San Vicente del Raspeig, E-03690 Alicante, Spain.; Multidisciplinary Institute for Environmental Studies 'Ramón Margalef', University of Alicante, Ap. 99, E-03080 Alicante, Spain. |
| Abstrakt: |
The haloarchaeon Haloferax mediterranei synthesizes poly(3-hydroxybutyrate- co -3-hydroxyvalerate) (PHBV) under unfavorable nutritional conditions without the addition of any precursor to the culture, which is an advantage compared to other microbial counterparts able to synthesize polyhydroxyalkanoates (PHA). PHBV is a biodegradable polymer showing physiochemical properties of biotechnological and biomedical interest and can be used as an alternative to plastics made from chemical synthesis (which are not environmentally friendly). The versatile metabolism of H. mediterranei makes the use of waste as a carbon source for cellular growth and PHA synthesis possible. In this work, cellular growth and the production and characterization of PHBV using two different types of confectionery waste were analyzed and compared with cellular growth and PHBV synthesis in a standard culture media with glucose of analytical grade as a carbon source. The PHBV granules produced were analyzed by TEM and the biopolymer was isolated and characterized by GC-MS, FTIR NMR, and DSC. The results reveal that H. mediterranei can use these two residues (R1 and R2) for pure PHBV production, achieving 0.256 and 0.983 g PHBV/L, respectively, which are among the highest yields so far described using for the first-time waste from the candy industry. Thus, a circular economy-based process has been designed to optimize the upscaling of PHBV production by using haloarchaea as cell factories and valorizing confectionery waste. |
