Oxygen matters: Unraveling the role of oxygen in the neuronal response to cisplatin.
| Autor: | Crugeiras J; Department of Cell Biology, Physiology, and Immunology, Institute of Neuroscience, Autonomous University of Barcelona, Bellaterra, Spain.; Biomedical Research Center Network on Neurodegenerative Diseases (CIBERNED), Bellaterra, Spain., Calls A; Department of Cell Biology, Physiology, and Immunology, Institute of Neuroscience, Autonomous University of Barcelona, Bellaterra, Spain.; Biomedical Research Center Network on Neurodegenerative Diseases (CIBERNED), Bellaterra, Spain., Contreras E; Department of Cell Biology, Physiology, and Immunology, Institute of Neuroscience, Autonomous University of Barcelona, Bellaterra, Spain.; Biomedical Research Center Network on Neurodegenerative Diseases (CIBERNED), Bellaterra, Spain., Alemany M; Unit of Neuro-Oncology, Hospital Universitari de Bellvitge, Bellvitge Institute for Biomedical Research (IDIBELL), Barcelona, Spain., Navarro X; Department of Cell Biology, Physiology, and Immunology, Institute of Neuroscience, Autonomous University of Barcelona, Bellaterra, Spain.; Biomedical Research Center Network on Neurodegenerative Diseases (CIBERNED), Bellaterra, Spain., Yuste VJ; Department of Biochemistry, Institute of Neuroscience, Autonomous University of Barcelona, Bellaterra, Spain., Casanovas O; Tumor Angiogenesis Group, ProCURE Program, Catalan Institute of Oncology, OncoBell Program, IDIBELL, Barcelona, Spain., Udina E; Department of Cell Biology, Physiology, and Immunology, Institute of Neuroscience, Autonomous University of Barcelona, Bellaterra, Spain.; Biomedical Research Center Network on Neurodegenerative Diseases (CIBERNED), Bellaterra, Spain., Bruna J; Biomedical Research Center Network on Neurodegenerative Diseases (CIBERNED), Bellaterra, Spain.; Unit of Neuro-Oncology, Hospital Universitari de Bellvitge, Bellvitge Institute for Biomedical Research (IDIBELL), Barcelona, Spain. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of the peripheral nervous system : JPNS [J Peripher Nerv Syst] 2024 Dec; Vol. 29 (4), pp. 528-536. Date of Electronic Publication: 2024 Sep 27. |
| DOI: | 10.1111/jns.12659 |
| Abstrakt: | Background and Aims: Cell culture is a fundamental experimental tool for understanding cell physiology. However, translating these findings to in vivo settings has proven challenging. Replicating donor tissue conditions, including oxygen levels, is crucial for achieving meaningful results. Nevertheless, oxygen culture conditions are often overlooked, particularly in the context of chemotherapy-induced neurotoxicity. Methods: In this study, we investigated the role of oxygen levels in primary neuronal cultures by comparing neuronal performance under cisplatin exposure (1 μg/mL) in supraphysiological normoxia (representing atmospheric conditions in a standard incubator; 18.5% O Results: Under control conditions, both oxygen concentration conditions exhibited similar behaviors. However, after cisplatin administration, sensory neurons cultured under supraphysiological normoxic conditions show higher mortality, exhibit an evolutionarily proinflammatory cytokine profile over time, and activate apoptotic-regulated neuron death markers. In contrast, under physiological conditions, neurons treated with cisplatin exhibited senescence marker expression and an attenuated inflammatory secretome. Interpretation: These results underscore the critical role of oxygen in neuronal culture, particularly in studying compounds where neuronal damage is mechanistically linked to oxidative stress. Even at identical doses of evaluated neurotoxic drugs, distinct cellular phenotypic fates can emerge, impacting translatability to the in vivo setting. (© 2024 The Author(s). Journal of the Peripheral Nervous System published by Wiley Periodicals LLC on behalf of Peripheral Nerve Society.) |
| Databáze: | MEDLINE |
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