Mitigation of Diabetes Mellitus Using Euphorbia helioscopia Leaf Ethanolic Extract by Modulating GCK, GLUT4, IGF, and G6P Expressions in Streptozotocin-Induced Diabetic Rats.

Autor: Raza A; Faculty of Pharmacy The University of Lahore, Lahore 54000, Pakistan., Mushtaq MN; Faculty of Pharmacy The University of Lahore, Lahore 54000, Pakistan., Hassan S; Department of Biomedical Engineering and Sciences School of Mechanical and Manufacturing Engineering National University of Science and Technology, Islamabad 24090, Pakistan., Sharif A; Department of Pharmacology Faculty of Pharmaceutical and Allied Health Sciences Lahore College for Women University, Lahore 54000, Pakistan., Akhtar B; Department of Pharmacy University of Agriculture, Faisalabad 38000, Pakistan., Akhtar MF; Riphah Institute of Pharmaceutical Sciences Riphah International University Lahore Campus, Lahore 54000, Pakistan.
Jazyk: angličtina
Zdroj: Journal of diabetes research [J Diabetes Res] 2024 Sep 18; Vol. 2024, pp. 5497320. Date of Electronic Publication: 2024 Sep 18 (Print Publication: 2024).
DOI: 10.1155/2024/5497320
Abstrakt: Diabetes mellitus is a metabolic disorder. Synthetic antidiabetics are the commonly used treatment options associated with complications. The objective of this study was to explore the antioxidative and antidiabetic potential of Euphorbia helioscopia whole plant ethanolic extract using in vitro and in vivo models. For that purpose, the antioxidative potential was explored by using 2,2-diphenyl-1-picrylhydrazyl analysis. In vitro antidiabetic potential of the extract was evaluated using amylase inhibitory analysis. In vivo antidiabetic activity of the extract was assessed in diabetic rats using streptozotocin/nicotinamide (60 mg/kg/120 mg/kg) as an inducing agent. Metformin was used as standard. The results indicated the presence of significant quantities of phenolic 82.18 ± 1.28 mgg -1 gallic acid equivalent (GAE) and flavonoid 66.55±1.22 mgg -1 quercetin equivalent (QE) contents in the extract. Quantitation of phytoconstituents exhibited the presence of sinapic acid, myricetin, and quercetin using HPLC analysis. The extract inhibited α -amylase by 84.71%, and an antiglycemic potential of 50.34% was assessed in the OGTT assay. Biochemical analysis demonstrated a reduction in urea, creatinine, cholesterol, low-density lipoprotein, and alkaline phosphatase ( p < 0.001) as compared to diabetic control rats at the dose of 500 mg/kg. An upregulation in the expressions of glucokinase, glucose transporter 4, peroxisome proliferator-activated receptor γ , and insulin-like growth factor was observed in treated rats in contrast to G6P expression, which was downregulated upon treatment. In conclusion, this study provided evidence of the antioxidative and antidiabetic potential of E. helioscopia whole plant ethanolic extract through in vitro and in vivo analysis and emphasized its promising role as a natural alternative.
Competing Interests: The authors declare no conflicts of interest.
(Copyright © 2024 Ahmed Raza et al.)
Databáze: MEDLINE
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