Bucking the trend: understanding lipopolysaccharide structure and outer membrane dynamics in cold-adapted Pseudomonas isolated from Enigma Lake, Antarctica.

Autor:	Mercogliano M; Department of Chemical Science, University of Naples Federico II Via Cinthia, 4 Naples 80126 Italy flaviana.dilorenzov@unina.it., De Chiara S; Department of Chemical Science, University of Naples Federico II Via Cinthia, 4 Naples 80126 Italy flaviana.dilorenzov@unina.it., De Nicola A; Cineca Casalecchio di Reno (BO) 40033 Italy adenicola.chem@gmail.com.; Research Center for Organic Electronics (ROEL), Yamagata University 4-3-16 Jonan Yonezawa Yamagata 992-8510 Japan., Cardellini J; Consorzio Interuniversitario Per Lo Sviluppo Dei Sistemi A Grande Interfase Via Della Lastruccia 3 Firenze 50019 Italy costanza.montis@unifi.it.; Dipartimento Di Chimica 'Ugo Schiff', Università Degli Studi di Firenze Via Della Lastruccia 3 Firenze 50019 Italy., Montis C; Consorzio Interuniversitario Per Lo Sviluppo Dei Sistemi A Grande Interfase Via Della Lastruccia 3 Firenze 50019 Italy costanza.montis@unifi.it.; Dipartimento Di Chimica 'Ugo Schiff', Università Degli Studi di Firenze Via Della Lastruccia 3 Firenze 50019 Italy., Yakimov MM; Institute of Polar Sciences, National Research Council of Italy (ISP-CNR) Via S. Raineri 4 Messina 98122 Italy., La Cono V; Institute of Polar Sciences, National Research Council of Italy (ISP-CNR) Via S. Raineri 4 Messina 98122 Italy., Crisafi F; Institute of Polar Sciences, National Research Council of Italy (ISP-CNR) Via S. Raineri 4 Messina 98122 Italy., Silipo A; Department of Chemical Science, University of Naples Federico II Via Cinthia, 4 Naples 80126 Italy flaviana.dilorenzov@unina.it.; CEINGE-Biotecnologie Avanzate Franco Salvatore Via Gaetano Salvatore 486 Napoli 80145 Italy., Berti D; Consorzio Interuniversitario Per Lo Sviluppo Dei Sistemi A Grande Interfase Via Della Lastruccia 3 Firenze 50019 Italy costanza.montis@unifi.it.; Dipartimento Di Chimica 'Ugo Schiff', Università Degli Studi di Firenze Via Della Lastruccia 3 Firenze 50019 Italy., Milano G; Department of Chemical, Material and Production Engineering, University of Naples Federico II Piazzale V. Tecchio, 80 Naples 80125 Italy., Molinaro A; Department of Chemical Science, University of Naples Federico II Via Cinthia, 4 Naples 80126 Italy flaviana.dilorenzov@unina.it.; CEINGE-Biotecnologie Avanzate Franco Salvatore Via Gaetano Salvatore 486 Napoli 80145 Italy.; Department of Chemistry, School of Science, Osaka University 1-1 Osaka University Machikaneyama Toyonaka Osaka 560-0043 Japan., Di Lorenzo F; Department of Chemical Science, University of Naples Federico II Via Cinthia, 4 Naples 80126 Italy flaviana.dilorenzov@unina.it.; CEINGE-Biotecnologie Avanzate Franco Salvatore Via Gaetano Salvatore 486 Napoli 80145 Italy.
Jazyk:	angličtina
Zdroj:	Chemical science [Chem Sci] 2024 Sep 18. Date of Electronic Publication: 2024 Sep 18.
DOI:	10.1039/d4sc05116e
Abstrakt:	Cold environments are predominant over the Earth and are inhabited by bacteria able to cope with a series of simultaneous environmental pressures. Gram-negative species of the Pseudomonas genus are the predominant ones isolated from cold habitats, making them an excellent model for studying the mechanisms of bacterial adaptation to the most extreme habitats on our planet. Here we focused on the lipopolysaccharide (LPS) structure and the outer membrane dynamics of Pseudomonas sp. EW#7, a strain isolated from Enigma Lake in Antarctica where, among other extreme characteristics, water temperature can reach 0.4 °C. We show that near-zero growth temperature mostly affects the LPS lipid A component. An uncommon tendency of decreasing lipid A secondary hydroxylation while increasing its phosphorylation degree was observed. This resulted in a faster lateral diffusion of lipid chains in the membrane and therefore in an enhancement of its fluctuations that guarantee membrane integrity and flexibility. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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