Verticillium dahliae Elicitor VdSP8 Enhances Disease Resistance Through Increasing Lignin Biosynthesis in Cotton.

Autor: Wang Y; State Key Laboratory of Crop Stress Resistance and High-Efficiency Production, Key Laboratory of Plant Protection Resources and Pest Integrated Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, and College of Plant Protection, Northwest A&F University, Yangling, China., Qin J; State Key Laboratory of Crop Stress Resistance and High-Efficiency Production, Key Laboratory of Plant Protection Resources and Pest Integrated Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, and College of Plant Protection, Northwest A&F University, Yangling, China., Wei M; State Key Laboratory of Crop Stress Resistance and High-Efficiency Production, Key Laboratory of Plant Protection Resources and Pest Integrated Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, and College of Plant Protection, Northwest A&F University, Yangling, China., Liao X; State Key Laboratory of Crop Stress Resistance and High-Efficiency Production, Key Laboratory of Plant Protection Resources and Pest Integrated Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, and College of Plant Protection, Northwest A&F University, Yangling, China., Shang W; State Key Laboratory of Crop Stress Resistance and High-Efficiency Production, Key Laboratory of Plant Protection Resources and Pest Integrated Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, and College of Plant Protection, Northwest A&F University, Yangling, China., Chen J; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China., Subbarao KV; Department of Plant Pathology, University of California, Davis, c/o Sam Farr United States Crop Improvement and Protection Research Center, Salinas, California, USA., Hu X; State Key Laboratory of Crop Stress Resistance and High-Efficiency Production, Key Laboratory of Plant Protection Resources and Pest Integrated Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, and College of Plant Protection, Northwest A&F University, Yangling, China.
Jazyk: angličtina
Zdroj: Plant, cell & environment [Plant Cell Environ] 2024 Sep 26. Date of Electronic Publication: 2024 Sep 26.
DOI: 10.1111/pce.15170
Abstrakt: Verticillium wilt caused by the soil-borne fungus Verticillium dahliae Kleb., is a destructive plant disease that instigates severe losses in many crops. Improving plant resistance to Verticillium wilt has been a challenge in most crops. In this study, a V. dahliae secreted protein VdSP8 was identified and shown to activate hyper-sensitive response (HR) and systemic acquired resistance (SAR) to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and Botrytis cinerea in tobacco plants. We identified a β-glucosidase named GhBGLU46 as a cotton plant target of VdSP8. VdSP8 interacts with GhBGLU46 both in vivo and in vitro and promotes the β-glucosidase activity of GhBGLU46. Silencing of GhBGLU46 reduced the expression of genes involved in lignin biosynthesis, such as GhCCR4, GhCCoAOMT2, GhCAD3 and GhCAD6, thus decreasing lignin deposition and increasing Verticillium wilt susceptibility. We have shown that GhBGLU46 is indispensable for the function of VdSP8 in plant resistance. These results suggest that plants have also evolved a strategy to exploit the invading effector protein VdSP8 to enhance plant resistance.
(© 2024 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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