Systemic ozone therapy as an adjunctive treatment in guided bone regeneration: a histomorphometrical and immunohistochemical study in rats.

Autor: Baggio AMP; Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), São Paulo, Brazil. ana.baggio@unesp.br., Bizelli VF; Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), São Paulo, Brazil., Delamura IF; Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), São Paulo, Brazil., Viotto AHA; Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), São Paulo, Brazil., Veras ASC; Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), São Paulo, Brazil., Teixeira GR; Multicentric Postgraduate Program in Physiological Sciences - SBFis, School of Dentistry, São Paulo State University (UNESP), Araçatuba, São Paulo, Brazil.; Department of Physical Education, School of Science and Technology, São Paulo State University (UNESP), Presidente Prudente, São Paulo, Brazil., Faverani LP; Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), São Paulo, Brazil., Bassi APF; Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Clinical oral investigations [Clin Oral Investig] 2024 Sep 27; Vol. 28 (10), pp. 556. Date of Electronic Publication: 2024 Sep 27.
DOI: 10.1007/s00784-024-05961-3
Abstrakt: Objectives: To assess the effectiveness of ozone therapy in guided bone regeneration (GBR) for critical size calvarial defects in rats.
Materials and Methods: 96 male Wistar rats were divided into four groups (n = 6 each). An 8 mm critical defect was created in the calvaria of each rat. The groups were: BIO (porcine collagen membrane, BioGide ® ), BIO + OZ (membrane with systemic ozone therapy every 2 days), COA + OZ (blood clot with ozone therapy), and COA (blood clot only). Evaluations at 7, 15, 30, and 60 days included histological, histomorphometric, inflammatory profile, Micro-CT, and immunohistochemical analyses. Statistical analysis involved two-factor ANOVA with Tukey's post-hoc test for general data, and one-factor ANOVA with Holm-Sidak post-hoc test for Micro-CT data.
Results: The BIO + OZ group demonstrated superior bone regeneration with well-organized, mature bone tissue and significant bone formation at 30 and 60 days. The COA + OZ group showed early angiogenesis and reduced inflammation, resulting in complete defect closure by 30 days. The BIO group had good regeneration, but less mature tissue compared to BIO + OZ. The COA group exhibited limited bone formation and higher porosity.
Conclusion: Ozone therapy positively influences bone regeneration by enhancing cell proliferation and the healing response.
Clinical Relevance: Improving regenerative processes with auxiliary therapies like ozone therapy can be significant for advancing dental reconstructions.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: