Human sperm mitochondrial DNA copy numbers and deletion rates: Comparing persons living in two urban industrial agglomerations differing in sources of air pollution.

Autor: Vozdova M; Department of Genetics and Reproductive Biotechnologies, Central European Institute of Technology - Veterinary Research Institute, Brno, Czech Republic. Electronic address: miluse.vozdova@vri.cz., Kubickova S; Department of Genetics and Reproductive Biotechnologies, Central European Institute of Technology - Veterinary Research Institute, Brno, Czech Republic. Electronic address: svatava.kubickova@vri.cz., Kopecka V; Department of Genetics and Reproductive Biotechnologies, Central European Institute of Technology - Veterinary Research Institute, Brno, Czech Republic. Electronic address: vera.kopecka@vri.cz., Sipek J; Department of Genetics and Reproductive Biotechnologies, Central European Institute of Technology - Veterinary Research Institute, Brno, Czech Republic. Electronic address: jaroslav.sipek@vri.cz., Rubes J; Department of Genetics and Reproductive Biotechnologies, Central European Institute of Technology - Veterinary Research Institute, Brno, Czech Republic. Electronic address: jiri.rubes@vri.cz.
Jazyk: angličtina
Zdroj: Mutation research. Genetic toxicology and environmental mutagenesis [Mutat Res Genet Toxicol Environ Mutagen] 2024 Oct; Vol. 899, pp. 503824. Date of Electronic Publication: 2024 Sep 08.
DOI: 10.1016/j.mrgentox.2024.503824
Abstrakt: Persons living in industrial environments are exposed to levels of air pollution that can affect their health and fertility. The Czech capital city, Prague, and the Ostrava industrial agglomeration differ in their major sources of air pollution. In Prague, heavy traffic produces high levels of nitrogen oxides throughout the year. In the Ostrava region, an iron industry and local heating are sources of particulate matter (PM) and benzo[a]pyrene (B[a]P), especially in the winter. We evaluated the effects of air pollution on human sperm mitochondrial DNA (mtDNA). Using real-time PCR, we analysed sperm mtDNA copy number and deletion rate in Prague city policemen in two seasons (spring and autumn) and compared the results with those from Ostrava. In Prague, the sperm mtDNA deletion rate was significantly higher in autumn than in spring, which is the opposite of the results from Ostrava. The sperm mtDNA copy number did not show any seasonal differences in either of the cities; it was correlated negatively with sperm concentration, motility, and viability, and with sperm chromatin integrity (assessed with the Sperm Chromatin Structure Assay). The comparison between the two cities showed that the sperm mtDNA deletion rate in spring and the sperm mtDNA copy number in autumn were significantly lower in Prague vs. Ostrava. Our study supports the hypothesis that sperm mtDNA deletion rate is affected by the composition of air pollution. Sperm mtDNA abundance is closely associated with chromatin damage and standard semen characteristics.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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