Chitosan/collagen biomembrane loaded with 2,3-dihydrobenzofuran for the treatment of cutaneous Leishmaniasis.

Autor: Braz EMA; Laboratório Interdisciplinar de Materiais Avançados-LIMAV, Universidade Federal do Piauí, Campus Ministro Petrônio Portella, Teresina, PI 64049-550, Brazil., Silva SCCC; Laboratório Interdisciplinar de Materiais Avançados-LIMAV, Universidade Federal do Piauí, Campus Ministro Petrônio Portella, Teresina, PI 64049-550, Brazil; Universidade Estadual do Piauí, Campus Professor Ariston Dias Lima, São Raimundo Nonato, PI 64770-000, Brazil., Alves MMM; Núcleo de Pesquisa em Plantas Medicinais-NPPM, Universidade Federal do Piauí, Campus Ministro Petrônio Portella, Teresina, PI 64049-550, Brazil; Departamento de Morfofisiologia Veterinária, Universidade Federal do Piauí, Campus Ministro Petrônio Portella, Teresina, PI 64049-550, Brazil., Carvalho FAA; Núcleo de Pesquisa em Plantas Medicinais-NPPM, Universidade Federal do Piauí, Campus Ministro Petrônio Portella, Teresina, PI 64049-550, Brazil., Magalhães R; Universidade Católica Portuguesa, CBQF-Centro de Biotecnologia e Química Fina Laboratório Associado, Escola Superior de Biotecnologia, Porto 4169-005, Portugal., Osajima JA; Laboratório Interdisciplinar de Materiais Avançados-LIMAV, Universidade Federal do Piauí, Campus Ministro Petrônio Portella, Teresina, PI 64049-550, Brazil., Silva DA; Laboratório Interdisciplinar de Materiais Avançados-LIMAV, Universidade Federal do Piauí, Campus Ministro Petrônio Portella, Teresina, PI 64049-550, Brazil., Oliveira AL; Universidade Católica Portuguesa, CBQF-Centro de Biotecnologia e Química Fina Laboratório Associado, Escola Superior de Biotecnologia, Porto 4169-005, Portugal., Muniz EC; Laboratório Interdisciplinar de Materiais Avançados-LIMAV, Universidade Federal do Piauí, Campus Ministro Petrônio Portella, Teresina, PI 64049-550, Brazil; Universidade Estadual de Maringá, Departamento de Química, Maringá, PR 87020-970, Brazil., Silva-Filho EC; Laboratório Interdisciplinar de Materiais Avançados-LIMAV, Universidade Federal do Piauí, Campus Ministro Petrônio Portella, Teresina, PI 64049-550, Brazil. Electronic address: edsonfilho@ufpi.edu.br.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Nov; Vol. 280 (Pt 4), pp. 135995. Date of Electronic Publication: 2024 Sep 24.
DOI: 10.1016/j.ijbiomac.2024.135995
Abstrakt: In this work, chitosan/collagen-based membranes loaded with 2,3-dihydrobenzofuran (2,3-DHB) were developed through a simple solvent-casting procedure for use in the treatment of cutaneous Leishmaniasis. The obtained membranes were characterized by elemental analysis, FTIR, TG, DSC, and XRD. Porosity, swelling, mechanical properties, hydrophilicity, and antioxidant activity were analyzed. In addition, assessment to the biocompatibility, through fibroblasts/keratinocytes and in vitro wound healing essays were performed. The obtained results show that the new 2,3-DHB loaded chitosan/collagen membrane presented high porosity and swelling capacity as well as maximum strength, hydrophilicity, and antioxidant activity higher in relation to the control. The tests of antileishmanial activity and the AFM images demonstrate great efficacy of inhibition growth of the parasite, superior to those from the standard therapeutic agent that is currently used: Amphotericin B. The new membranes are biocompatible and stimulated the proliferation of keratinocytes. SEM images clearly demonstrate that fibroblasts were able to adhere, maintained their characteristic morphology. The healing test evidenced that the membranes have adequate environment for promoting cell proliferation and growth. As the conventional treatments often use drugs with high toxicity, the as-developed new membranes proved to be excellent candidate to treat cutaneous Leishmaniasis and can be clearly indicated for further advanced studies in vivo.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Edvani Curti Muniz reports financial support was provided by National Council for Scientific and Technological Development. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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