Lactobacillus plantarum NCHBL-004 modulates high-fat diet-induced weight gain and enhances GLP-1 production for blood glucose regulation.

Autor: Jang AR; Laboratory Animal Medicine, College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju 61186, Republic of Korea; Nodcure, INC., 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of Korea., Jung DH; Laboratory Animal Medicine, College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju 61186, Republic of Korea., Lee TS; Laboratory Animal Medicine, College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju 61186, Republic of Korea., Kim JK; School of Pharmacy and Institute of New Drug Development, Jeonbuk National University, Jeonju 54896, Republic of Korea., Lee YB; School of Pharmacy and Institute of New Drug Development, Jeonbuk National University, Jeonju 54896, Republic of Korea., Lee JY; Nodcure, INC., 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of Korea., Kim SY; Department of Microbiology, College of Medicine, Konyang University, Daejeon, Republic of Korea., Yoo YC; Department of Microbiology, College of Medicine, Konyang University, Daejeon, Republic of Korea., Ahn JH; Department of Pharmacy, Kangwon National University, Chuncheon 24341, Republic of Korea; KNU Researcher training program for Innovative Drug Development Research Team for Intractable Diseases (BK21 plus), Kangwon National University, Chuncheon 24341, Republic of Korea; Global/Gangwon Innovative Biologics-Regional Leading Research Center (GIB-RLRC), Kangwon National University, Chuncheon 24341, Republic of Korea., Hong EH; Department of Pharmacy, Kangwon National University, Chuncheon 24341, Republic of Korea; KNU Researcher training program for Innovative Drug Development Research Team for Intractable Diseases (BK21 plus), Kangwon National University, Chuncheon 24341, Republic of Korea; Global/Gangwon Innovative Biologics-Regional Leading Research Center (GIB-RLRC), Kangwon National University, Chuncheon 24341, Republic of Korea., Kim CW; Department of Pharmacy, Kangwon National University, Chuncheon 24341, Republic of Korea; KNU Researcher training program for Innovative Drug Development Research Team for Intractable Diseases (BK21 plus), Kangwon National University, Chuncheon 24341, Republic of Korea; Global/Gangwon Innovative Biologics-Regional Leading Research Center (GIB-RLRC), Kangwon National University, Chuncheon 24341, Republic of Korea., Kim SM; Pharmacomicrobiomics Research Center, College of Pharmacy, Hanyang University, Ansan, Gyeonggi-do 15588, Republic of Korea., Yoo HH; Pharmacomicrobiomics Research Center, College of Pharmacy, Hanyang University, Ansan, Gyeonggi-do 15588, Republic of Korea., Huh JY; College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea., Ko HJ; Department of Pharmacy, Kangwon National University, Chuncheon 24341, Republic of Korea; KNU Researcher training program for Innovative Drug Development Research Team for Intractable Diseases (BK21 plus), Kangwon National University, Chuncheon 24341, Republic of Korea; Global/Gangwon Innovative Biologics-Regional Leading Research Center (GIB-RLRC), Kangwon National University, Chuncheon 24341, Republic of Korea., Park JH; Laboratory Animal Medicine, College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju 61186, Republic of Korea; Nodcure, INC., 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of Korea. Electronic address: jonpark@jnu.ac.kr.
Jazyk: angličtina
Zdroj: Nutrition (Burbank, Los Angeles County, Calif.) [Nutrition] 2024 Dec; Vol. 128, pp. 112565. Date of Electronic Publication: 2024 Aug 29.
DOI: 10.1016/j.nut.2024.112565
Abstrakt: Objectives: This study investigated the therapeutic potential of Lactobacillus plantarum NCHBL-004 (NCHBL-004) in the treatment of obesity and associated metabolic disorders.
Methods: Mice were fed either a normal diet (ND) or a high-fat diet (HFD) with oral administration of NCHBL-004. After euthanasia, blood, liver and adipose tissue were collected. Furthermore, the microbiome and short-chain fatty acids (SCFAs) were analyzed from feces.
Results: Oral administration of live NCHBL-004 to mice fed a HFD resulted in notable reductions in weight gain, improvements in glucose metabolism, and maintenance of balanced lipid levels. A comparative analysis with other Lactobacillus strains highlighted the superior efficacy of NCHBL-004. Moreover, heat-killed NCHBL-004 demonstrated beneficial effects similar to those of live NCHBL-004. Additionally, administration of live NCHBL-004 induced glucagon-like peptide 1 (GLP-1) production and increased the levels of short-chain fatty acids (SCFAs), including acetate and propionate, in feces, positively influencing liver lipid metabolism and mitigating inflammation. Consistent with this, analysis of the gut microbiome following NCHBL-004 administration showed increases in SCFA-producing microbes with increased proportions of Lactobacillus spp. and a significant increase in the proportion of microbes capable of promoting GLP-1 secretion.
Conclusions: These findings underscore the potential of both live and inactivated NCHBL-004 as potential therapeutic approaches to managing obesity and metabolic disorders, suggesting avenues for further investigation and clinical applications.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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