Nanomolar Hg 2+ detection via phenothiazine fluorogenic chemosensor: Applications in water analysis and intracellular imaging.

Autor: Ilakiyalakshmi M; Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore 632014, Tamil Nadu, India., Mohandoss S; School of Chemical Engineering, Yeungnam University, Gyeongsan, Gyeonbuk-do 38541, Republic of Korea., Mohana Roopan S; Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore 632014, Tamil Nadu, India., Arumugam Napoleon A; Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore 632014, Tamil Nadu, India. Electronic address: aanapoleon@vit.ac.in.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2025 Feb 05; Vol. 326, pp. 125157. Date of Electronic Publication: 2024 Sep 21.
DOI: 10.1016/j.saa.2024.125157
Abstrakt: A Knoevenagel condensation reaction paved the way for the development of the PTZ-BCN probe namely (z)-2-(1H-benzo[d]imidazole-2-yl)-3-(10-ethyl-10H-phenothiazin-3-yl) acrylonitrile. PTZ-BCN's spectral characteristics were verified through the application of IR, 1 H NMR, 13 C NMR, and HRMS techniques. The PTZ-BCN probe showed a high selectivity and sensitivity toward Hg 2+ ions over other interfering competing metal ions in CH 3 CN: HEPES buffer (9:1, v/v) system. The addition of Hg 2+ results in a notable redshift and fluorescence quenching of the PTZ-BCN probe. An examination of the interaction between the PTZ-BCN probe and Hg 2+ involved recoding 1 H NMR titration spectra, HRMS, DFT analysis, and Job's plot respectively. Quantification of the lowest detectable Hg 2+ concentration at 2.3 nM, exhibiting a correlation coefficient of R 2  = 0.9985. PTZ-BCN probe has proven effective in quantitatively determining the existence of Hg 2+ in real-time water samples, test strips, solid state, and Hela living cells.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
Externí odkaz: