Comparative analyses of the transcriptome among three development stages of Zeugodacus tau larvae (Diptera: Tephritidae).

Autor: Li WJ; Department of Plant Protection, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China; Nanchang Key Laboratory of Surveillance and Management for Horticultural Crop Pests, Jiangxi Agricultural University, Nanchang 330045, China., Xu CK; Department of Plant Protection, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China., Ong SQ; Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu, Sabah 88400, Malaysia., Majid AHA; Household and Structural Urban Entomology Laboratory, Vector Control Research Unit, School of Biological Sciences, Universiti Sains Malaysia, Penang 11800, Malaysia., Wang JG; Department of Plant Protection, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China., Li XZ; Department of Plant Protection, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China; Nanchang Key Laboratory of Surveillance and Management for Horticultural Crop Pests, Jiangxi Agricultural University, Nanchang 330045, China. Electronic address: lxziiz@163.com.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Part D, Genomics & proteomics [Comp Biochem Physiol Part D Genomics Proteomics] 2024 Dec; Vol. 52, pp. 101333. Date of Electronic Publication: 2024 Sep 23.
DOI: 10.1016/j.cbd.2024.101333
Abstrakt: Studying differences in transcriptomes across various development stages of insects is necessary to uncover the physiological and molecular mechanism underlying development and metamorphosis. We here present the first transcriptome data generated under Illumina Hiseq platform concerning Zeugodacus tau (Walker) larvae from Nanchang, China. In total, 11,702 genes were identified from 9 transcriptome libraries of three development stages of Z. tau larvae. 7219 differentially expressed genes (DEGs) were screened out from the comparisons between each two development stages of Z. tau larvae, and their roles in development and metabolism were analyzed. Comparative analyses of transcriptome data showed that there are 5333 DEGs between 1-day and 7-day old larvae, consisting of 1609 up-regulated and 3724 down-regulated genes. Expressions of DEGs were more abundant in L7 than in L1 and L3, which might be associated with metamorphosis. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis suggested the enrichment of metabolic process. KOG annotation further confirmed that 20-hydroxyecdysone (20E) pathway related genes Cyp4ac1_1, Cyp4aa1, Cyp313a4_3 were critical for the biosynthesis, transport, and catabolism of secondary metabolites and lipid transport and metabolism. Expression patterns of 8 DEGs were verified using quantitative real-time PCR (RT-qPCR). This study elucidated the DEGs and their roles underlying three development stages of Z. tau larvae, which provided valuable information for further functional genomic research.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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