Biosensing strategies using recombinant luminescent proteins and their use for food and environmental analysis.

Autor: Pradanas-González F; Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Plaza Ciencias 2, 28040, Madrid, Spain., Cortés MG; Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Plaza Ciencias 2, 28040, Madrid, Spain., Glahn-Martínez B; Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Plaza Ciencias 2, 28040, Madrid, Spain., Del Barrio M; Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Plaza Ciencias 2, 28040, Madrid, Spain., Purohit P; Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Plaza Ciencias 2, 28040, Madrid, Spain. ppurohit@ucm.es., Benito-Peña E; Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Plaza Ciencias 2, 28040, Madrid, Spain. elenabp@ucm.es., Orellana G; Department of Organic Chemistry, Faculty of Chemistry, Complutense University of Madrid, Plaza Ciencias 2, 28040, Madrid, Spain.
Jazyk: angličtina
Zdroj: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2024 Dec; Vol. 416 (30), pp. 7205-7224. Date of Electronic Publication: 2024 Sep 26.
DOI: 10.1007/s00216-024-05552-x
Abstrakt: Progress in synthetic biology and nanotechnology plays at present a major role in the fabrication of sophisticated and miniaturized analytical devices that provide the means to tackle the need for new tools and methods for environmental and food safety. Significant research efforts have led to biosensing experiments experiencing a remarkable growth with the development and application of recombinant luminescent proteins (RLPs) being at the core of this boost. Integrating RLPs into biosensors has resulted in highly versatile detection platforms. These platforms include luminescent enzyme-linked immunosorbent assays (ELISAs), bioluminescence resonance energy transfer (BRET)-based sensors, and genetically encoded luminescent biosensors. Increased signal-to-noise ratios, rapid response times, and the ability to monitor dynamic biological processes in live cells are advantages inherent to the approaches mentioned above. Furthermore, novel fusion proteins and optimized expression systems to improve their stability, brightness, and spectral properties have enhanced the performance and pertinence of luminescent biosensors in diverse fields. This review highlights recent progress in RLP-based biosensing, showcasing their implementation for monitoring different contaminants commonly found in food and environmental samples. Future perspectives and potential challenges in these two areas of interest are also addressed, providing a comprehensive overview of the current state and a forecast of the biosensing strategies using recombinant luminescent proteins to come.
Competing Interests: Declarations. Conflict of interest: The authors declare no competing interests. Elena Benito-Peña and Guillermo Orellana are guest editors for this topical collection in ABC but were not involved in the peer review of this paper.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.)
Databáze: MEDLINE
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