K 2P 2.1 channels modulate the pH- and mechanosensitivity of pancreatic stellate cells.

Autor: Rugi M; Institut Für Physiologie II, Robert-Koch-Str. 27B, 48149, Münster, Germany., Hofschröer V; Institut Für Physiologie II, Robert-Koch-Str. 27B, 48149, Münster, Germany., Pethő Z; Institut Für Physiologie II, Robert-Koch-Str. 27B, 48149, Münster, Germany., Soret B; Institut Für Physiologie II, Robert-Koch-Str. 27B, 48149, Münster, Germany.; Laboratory of Cell Physiology, INSERM U 1003, Laboratory of Excellence Ion Channel Science and Therapeutics, Department of Biology, Faculty of Science and Technologies, University of Lille, 59650, Villeneuve d'Ascq, France., Loeck T; Institut Für Physiologie II, Robert-Koch-Str. 27B, 48149, Münster, Germany., Schwab A; Institut Für Physiologie II, Robert-Koch-Str. 27B, 48149, Münster, Germany. aschwab@uni-muenster.de.
Jazyk: angličtina
Zdroj: Pflugers Archiv : European journal of physiology [Pflugers Arch] 2024 Sep 26. Date of Electronic Publication: 2024 Sep 26.
DOI: 10.1007/s00424-024-03021-z
Abstrakt: Pancreatic stellate cells (PSCs) are central in the development of acute pancreatitis and tumor fibrosis in pancreatic ductal adenocarcinoma (PDAC). Fibrosis and a unique pH landscape represent characteristic properties of the PDAC microenvironment. Mechanosensitive ion channels are involved in the activation of PSCs. Among these channels, K 2P 2.1 has not yet been studied in PSCs. K 2P 2.1 channels are pH- and mechanosensitive. We confirmed K 2P 2.1 expression in PSCs by RT-qPCR and immunofluorescence. PSCs from K 2P 2.1 +/+ and K 2P 2.1 -/- mice were studied under conditions mimicking properties of the PDAC microenvironment (acidic extracellular pH (pH e ), ambient pressure elevated by + 100 mmHg). Migration and the cell area were taken as surrogates for PSC activation and evaluated with live cell imaging. pH e -dependent changes of the membrane potential of PSCs were investigated with DiBAC 4 (3), a voltage-sensitive fluorescent dye. We observed a correlation between morphological activation and progressive hyperpolarization of the cells in response to changes in pH e and pressure. The effect was in part dependent on the expression of K 2P 2.1 channels because the membrane potential of K 2P 2.1 +/+ PSCs was always more hyperpolarized than that of K 2P 2.1 -/- PSCs. Cell migration velocity of K 2P 2.1 +/+ cells decreased upon pressure application when cells were kept in an acidic medium (pH e 6.6). This was not the case in K 2P 2.1 -/- PSCs. Taken together, our study highlights the critical role of K 2P 2.1 channels in the combined sensing of environmental pressure and pH e by PSCs and in coordinating cellular morphology with membrane potential dynamics. Thus, K 2P 2.1 channels are important mechano-sensors in murine PSCs.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz: