Nanosensor based on HP-MAP1 and carbon nanotubes for bacteria detection.

Autor: Silva WFSM; Programa de Pós-Graduação em Inovação Terapêutica, Universidade Federal de Pernambuco, Recife, Brazil., Migliolo L; S-Inova Biotech, Programa de Pós-graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, Brazil., Silva PS; S-Inova Biotech, Programa de Pós-graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, Brazil., Lima GMS; Departamento de Antibióticos, Universidade Federal de Pernambuco, Recife, Brazil., Oliveira MDL; Programa de Pós-Graduação em Inovação Terapêutica, Universidade Federal de Pernambuco, Recife, Brazil.; Laboratório de Biodispositivos Nanoestruturados, Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Brazil., Andrade CAS; Programa de Pós-Graduação em Inovação Terapêutica, Universidade Federal de Pernambuco, Recife, Brazil.; Laboratório de Biodispositivos Nanoestruturados, Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Brazil.
Jazyk: angličtina
Zdroj: Biotechnology progress [Biotechnol Prog] 2024 Sep 26, pp. e3510. Date of Electronic Publication: 2024 Sep 26.
DOI: 10.1002/btpr.3510
Abstrakt: Healthcare-associated infections (HAIs) pose significant challenges to global health due to pathogen complexity and antimicrobial resistance. Biosensors utilizing antimicrobial peptides offer innovative solutions. Hylarana picturata Multiple Active Peptide 1 (Hp-MAP1), derived from Temporin-PTA, exhibits antibacterial properties sourced from the skin secretions of the Malaysian fire-bellied frog. An innovative sensing layer was developed for the electrochemical biorecognition of diverse pathogens: Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, and Staphylococcus aureus. Electrochemical impedance spectroscopy differentiated microorganisms based on distinct electrochemical responses. The sensor layer, composed of functionalized multi-walled carbon nanotubes (MWCNTs) associated with Hp-MAP1, exhibited varying levels of charge transfer resistance (R CT ) for different microorganisms. Gram-negative species, especially P. aeruginosa, displayed higher R CT values, indicating better impedimetric responses. Excellent LODs were observed for P. aeruginosa (0.60), K. pneumoniae (0.42), E. coli (0.67), and S. aureus (0.59), highlighting the efficacy of the MWCNTs/Hp-MAP1 biosensor in microbial identification. The MWCNTs/Hp-MAP1 biosensor platform presents a promising and effective microbial identification strategy with potential healthcare applications to mitigate HAIs and enhance patient care.
(© 2024 American Institute of Chemical Engineers.)
Databáze: MEDLINE
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