ReBiA-Robotic Enabled Biological Automation: 3D Epithelial Tissue Production.

Autor: Königer L; Translational Center Regenerative Therapies, Fraunhofer Institute for Silicate Research ISC, 97070, Würzburg, Germany., Malkmus C; Translational Center Regenerative Therapies, Fraunhofer Institute for Silicate Research ISC, 97070, Würzburg, Germany.; Institute of Medical Engineering Schweinfurt, Technical University of Applied Sciences Würzburg-Schweinfurt, 97421, Schweinfurt, Germany., Mahdy D; Chair of Tissue Engineering and Regenerative Medicine, University Hospital Würzburg, 97070, Würzburg, Germany., Däullary T; Chair of Tissue Engineering and Regenerative Medicine, University Hospital Würzburg, 97070, Würzburg, Germany.; Chair of Cellular Immunotherapy, University Hospital Würzburg, 97080, Würzburg, Germany., Götz S; Faculty of Design Würzburg, Technical University of Applied Sciences Würzburg-Schweinfurt, 97070, Würzburg, Germany., Schwarz T; Translational Center Regenerative Therapies, Fraunhofer Institute for Silicate Research ISC, 97070, Würzburg, Germany., Gensler M; Chair of Tissue Engineering and Regenerative Medicine, University Hospital Würzburg, 97070, Würzburg, Germany., Pallmann N; Chair of Tissue Engineering and Regenerative Medicine, University Hospital Würzburg, 97070, Würzburg, Germany., Cheufou D; Department of Thoracic Surgery, Klinikum Würzburg Mitte, 97070, Würzburg, Germany., Rosenwald A; Institute of Pathology, University of Würzburg, 97080, Würzburg, Germany., Möllmann M; Translational Center Regenerative Therapies, Fraunhofer Institute for Silicate Research ISC, 97070, Würzburg, Germany., Groneberg D; Translational Center Regenerative Therapies, Fraunhofer Institute for Silicate Research ISC, 97070, Würzburg, Germany., Popp C; Translational Center Regenerative Therapies, Fraunhofer Institute for Silicate Research ISC, 97070, Würzburg, Germany., Groeber-Becker F; Translational Center Regenerative Therapies, Fraunhofer Institute for Silicate Research ISC, 97070, Würzburg, Germany.; Department of Ophthalmology, University Clinic Düsseldorf, 40225, Düsseldorf, Germany., Steinke M; Translational Center Regenerative Therapies, Fraunhofer Institute for Silicate Research ISC, 97070, Würzburg, Germany.; Department of Oto-Rhino-Laryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, University Hospital Würzburg, 97080, Würzburg, Germany., Hansmann J; Translational Center Regenerative Therapies, Fraunhofer Institute for Silicate Research ISC, 97070, Würzburg, Germany.; Institute of Medical Engineering Schweinfurt, Technical University of Applied Sciences Würzburg-Schweinfurt, 97421, Schweinfurt, Germany.
Jazyk: angličtina
Zdroj: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2024 Dec; Vol. 11 (45), pp. e2406608. Date of Electronic Publication: 2024 Sep 26.
DOI: 10.1002/advs.202406608
Abstrakt: The Food and Drug Administration's recent decision to eliminate mandatory animal testing for drug approval marks a significant shift to alternative methods. Similarly, the European Parliament is advocating for a faster transition, reflecting public preference for animal-free research practices. In vitro tissue models are increasingly recognized as valuable tools for regulatory assessments before clinical trials, in line with the 3R principles (Replace, Reduce, Refine). Despite their potential, barriers such as the need for standardization, availability, and cost hinder their widespread adoption. To address these challenges, the Robotic Enabled Biological Automation (ReBiA) system is developed. This system uses a dual-arm robot capable of standardizing laboratory processes within a closed automated environment, translating manual processes into automated ones. This reduces the need for process-specific developments, making in vitro tissue models more consistent and cost-effective. ReBiA's performance is demonstrated through producing human reconstructed epidermis, human airway epithelial models, and human intestinal organoids. Analyses confirm that these models match the morphology and protein expression of manually prepared and native tissues, with similar cell viability. These successes highlight ReBiA's potential to lower barriers to broader adoption of in vitro tissue models, supporting a shift toward more ethical and advanced research methods.
(© 2024 The Author(s). Advanced Science published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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