Genetic profiles and phenotypic patterns in Taiwanese Phalaenopsis orchids: a two-step phenotype and genotype strategy using modified genetic distance algorithms.

Autor:	Lai YS; Department of Agronomy, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan., Chen SY; Department of Agronomy, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan.; Orchid Research and Development Center, National Cheng Kung University, Tainan, Taiwan., Wu YJ; Orchid Research and Development Center, National Cheng Kung University, Tainan, Taiwan.; Department of Life Sciences, National Cheng Kung University, Tainan, Taiwan., Chen WH; Orchid Research and Development Center, National Cheng Kung University, Tainan, Taiwan., Chen HH; Orchid Research and Development Center, National Cheng Kung University, Tainan, Taiwan.; Department of Life Sciences, National Cheng Kung University, Tainan, Taiwan., Lin YY; Research and Development Department, Brother Orchid Nursery Co., Ltd., Taichung, Taiwan., Lin TC; Research and Development Department, Wonderorchids Co., Ltd., Taichung, Taiwan., Lin TJ; Research and Development Department, Wonderorchids Co., Ltd., Taichung, Taiwan., Kao CF; Department of Agronomy, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan.; Orchid Research and Development Center, National Cheng Kung University, Tainan, Taiwan.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2024 Sep 11; Vol. 15, pp. 1416886. Date of Electronic Publication: 2024 Sep 11 (Print Publication: 2024).
DOI:	10.3389/fpls.2024.1416886
Abstrakt:	This study establishes the first core collection (CC) for Taiwanese Phalaenopsis orchids to preserve genetic diversity and key traits essential for breeding and research, thereby enhancing breeding efficiency without the need for a large maintained parent population. We examined 207 commercial orchid cultivars from ten nurseries, characterized by two phenotypes and genotypic data from eight simple sequence repeat markers. Multiple imputation was applied to estimate missing phenotypes, minimizing potential uncertainties and ensuring the reliability of population structure analysis. Weighted k -means clustering identified seven distinct clusters, highlighting substantial genetic diversity. We proposed a two-step phenotype and genotype strategy and modified genetic distance algorithms to effectively preserve both phenotypic and genetic diversity while retaining key features. Consequently, 22 core accessions were selected, distributed across seven clusters, and representing the orchid germplasm collection. Our evaluation revealed significant diversity preservation, particularly in distinct characteristics and rare features, outperforming other methodologies. Pedigree background analysis further confirmed the representativeness of the CC in maintaining diverse genetic materials. We emphasized the importance of evaluating the CC by detailing the criteria and statistical analyses used to ensure the quality, representativeness, and effectiveness of the selected accessions. This study contributes to orchid breeding, conservation efforts, and sustainable agricultural practices by providing a valuable and comprehensive resource. In conclusion, our research establishes a groundbreaking CC, offering insights into the genetic landscape of Taiwanese Phalaenopsis orchids and highlighting potential advancements in breeding commercially desirable varieties. Competing Interests: Y-YL was employed by Brother Orchid Nursery Co., Ltd., T-CL and T-JL were employed by Wonderorchids Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Lai, Chen, Wu, Chen, Chen, Lin, Lin, Lin and Kao.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu