Proteomic exploration of potential blood biomarkers in haemophilic arthropathy.

Autor: Kalebota N; Department of Rheumatology and Rehabilitation University Hospital Centre Zagreb Zagreb Croatia., Novak R; Centre for Translational and Clinical Research, Department of Proteomics University of Zagreb, School of Medicine Zagreb Croatia.; BIMIS - Biomedical Research Centre Šalata University of Zagreb School of Medicine Zagreb Croatia., Hrkač S; Department of Clinical Immunology Allergology and Rheumatology, University Hospital Dubrava Zagreb Croatia., Perić P; Department of Rheumatology and Rehabilitation University Hospital Centre Zagreb Zagreb Croatia.; University of Zagreb, School of Medicine Zagreb Croatia., Salai G; Department of Pulmonology University Hospital Dubrava Zagreb Croatia., Močibob M; Department of Chemistry University of Zagreb, Faculty of Science Zagreb Croatia., Pranjić M; Department of Chemistry University of Zagreb, Faculty of Science Zagreb Croatia., Zdráhal Z; Central European Institute of Technology Masaryk University, Kamenice 5 Brno Czech Republic., Pustka V; Central European Institute of Technology Masaryk University, Kamenice 5 Brno Czech Republic., Žerjavić NL; Department of Rheumatology and Rehabilitation University Hospital Centre Zagreb Zagreb Croatia.; University of Zagreb, School of Medicine Zagreb Croatia., Milošević M; Andrija Štampar School of Public Health University of Zagreb, School of Medicine Zagreb Croatia., Vodanović M; Division of Hematology, Department of Internal Medicine University Hospital Centre Zagreb Zagreb Croatia.; University of Applied Health Sciences Zagreb Croatia., Šalek SZ; Department of Hematology Clinical Hospital 'Sv. Duh' Zagreb Croatia., Grgurević L; Centre for Translational and Clinical Research, Department of Proteomics University of Zagreb, School of Medicine Zagreb Croatia.; BIMIS - Biomedical Research Centre Šalata University of Zagreb School of Medicine Zagreb Croatia.; Department of Anatomy, 'Drago Perović' University of Zagreb, School of Medicine Zagreb Croatia.
Jazyk: angličtina
Zdroj: Health science reports [Health Sci Rep] 2024 Sep 25; Vol. 7 (9), pp. e70046. Date of Electronic Publication: 2024 Sep 25 (Print Publication: 2024).
DOI: 10.1002/hsr2.70046
Abstrakt: Background and Aims: The pathophysiology of haemophilic arthropathy (HA) is complex and largely undefined. Proteomic analyses provide insights into the intricate mechanisms of the HA.Our study aimed to identify differentially expressed proteins in relation to the severity of HA, explore their pathophysiological roles, and evaluate their potential as HA biomarkers.
Methods: Our cross-sectional observational study encompassed 30 HA patients and 15 healthy subjects. Plasma samples were pooled into three groups of 15 samples from those with severe haemophilic arthropathy (sHA), mild haemophilic arthropathy (mHA) and healthy controls. Proteomic analysis was performed using liquid chromatography-mass spectrometry. The severity of HA was assessed using the World Federation of Haemophilia Physical Examination Score and ultrasonography following the Haemophilia Early Arthropathy Detection with Ultrasound (HEAD-US) guidelines.
Results: A total of 788 proteins were identified, with 97% of the uniquely identified proteins being expressed in all analysed groups. We identified several up and downregulated proteins across the groups that were mainly related to inflammatory and immunity-modulating processes, as well as joint degeneration. We highlighted ten proteins relevant for the development of HA: cathepsin G, endoplasmic reticulum aminopeptidase 2, S100-A9, insulin-like growth factor I, apolipoprotein (a), osteopontin, pregnancy zone protein, cartilage oligomeric matrix protein, CD44, and cadherin-related family member 2.
Conclusion: Our analysis identified several proteins that shed further light on the distinctive pathogenesis of HA and could serve for biomarker research. However, these results need to be validated on a larger patient group.
Competing Interests: The authors declare no conflict of interest.
(© 2024 The Author(s). Health Science Reports published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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