Phenotypic and genotypic identification of class C and D β-lactamases in clinical isolates of Pseudomonas aeruginosa : a cross-sectional study.

Autor: Mohammadnezhad N; Department of Microbiology, School of Medicine, Tehran Medical Sciences Islamic Azad University Tehran Iran., Nazari M; Department of Microbiology, School of Medicine Ardabil University of Medical Sciences Ardabil Iran., Mostafavi SKS; Department of Microbiology, School of Medicine, Tehran Medical Sciences Islamic Azad University Tehran Iran., Sahebkar A; Center for Global Health Research, Saveetha Medical College and Hospitals, Saveetha Institute of Medical and Technical Sciences Saveetha University Chennai India.; Applied Biomedical Research Center Mashhad University of Medical Sciences Mashhad Iran.; Biotechnology Research Center, Pharmaceutical Technology Institute Mashhad University of Medical Sciences Mashhad Iran., Khademi F; Department of Microbiology, School of Medicine Ardabil University of Medical Sciences Ardabil Iran.; Arthropod-Borne Diseases Research Center Ardabil University of Medical Sciences Ardabil Iran.
Jazyk: angličtina
Zdroj: Health science reports [Health Sci Rep] 2024 Sep 24; Vol. 7 (9), pp. e70095. Date of Electronic Publication: 2024 Sep 24 (Print Publication: 2024).
DOI: 10.1002/hsr2.70095
Abstrakt: Background and Aims: The emergence of Pseudomonas aeruginosa ( P. aeruginosa ) antibiotic resistance is an important public health problem worldwide that can negatively affect infection control. Therefore, obtaining knowledge about antibiotic resistance mechanisms is necessary for infection control policies. This study aimed to determine the frequency of class C and D β-lactamases in P. aeruginosa strains isolated from patients referred to Ardabil hospitals using phenotypic and genotypic tests.
Methods: Phenotypic detection of β-lactamases including AmpC cephalosporinase, oxacillinase (OXA)-type extended-spectrum β-lactamases (ESBLs), and OXA-type carbapenemases were performed using the disk diffusion-based methods. Amplification of genes encoding classes C ( ampC and FOX genes) and D ( OXA-1 , OXA-2 , OXA-10 , OXA-23 , and OXA-48 genes) β-lactamases was performed using the polymerase chain reaction (PCR) method. A quantitative reverse transcription PCR (qRT-PCR) method was used to determine the expression level of the ampC gene among multiple drug-resistant and imipenem-resistant P. aeruginosa strains.
Results: In phenotypic tests, the prevalence of AmpC cephalosporinase, OXA-type ESBLs, and OXA-type carbapenemases were 52.5%, 7.2%, and 95.8%, respectively. In genotypic tests, the prevalence of ampC , FOX , OXA-1 , OXA-2 , OXA-10 , OXA-23 , and OXA-48 genes were 100%, 0%, 4.3%, 60.8%, 42%, 29.7%, and 2.9%, respectively. In addition, the ampC gene overexpression was seen in 16 (33.3%) drug-resistant P. aeruginosa clinical isolates.
Conclusion: Given the presence of class C and D β-lactamases in clinical isolates of P. aeruginosa in Ardabil hospitals, early detection of these strains can help prevent the spread of resistant strains in hospital environments and subsequent treatment failure.
Competing Interests: The authors declare that there is no conflict of interest.
(© 2024 The Author(s). Health Science Reports published by Wiley Periodicals LLC.)
Databáze: MEDLINE
Externí odkaz: