A novel nabelschnur protein regulates segregation of the kinetoplast DNA in Trypanosoma brucei.

Autor: Cadena LR; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis) 370 05, Czech Republic. Electronic address: lawrence.cadena@hhu.de., Hammond M; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis) 370 05, Czech Republic; Faculty of Sciences, University of South Bohemia, České Budějovice (Budweis) 370 05, Czech Republic. Electronic address: michael.hammond@paru.cas.cz., Tesařová M; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis) 370 05, Czech Republic., Chmelová Ľ; Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava 701 03, Czech Republic., Svobodová M; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis) 370 05, Czech Republic., Durante IM; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis) 370 05, Czech Republic., Yurchenko V; Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava 701 03, Czech Republic., Lukeš J; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis) 370 05, Czech Republic; Faculty of Sciences, University of South Bohemia, České Budějovice (Budweis) 370 05, Czech Republic.
Jazyk: angličtina
Zdroj: Current biology : CB [Curr Biol] 2024 Oct 21; Vol. 34 (20), pp. 4803-4812.e3. Date of Electronic Publication: 2024 Sep 24.
DOI: 10.1016/j.cub.2024.08.044
Abstrakt: The acquisition of mitochondria was imperative for initiating eukaryogenesis and thus is a characteristic feature of eukaryotic cells. 1 , 2 The parasitic protist Trypanosoma brucei contains a singular mitochondrion with a unique mitochondrial genome, termed the kinetoplast DNA (kDNA). 3 Replication of the kDNA occurs during the G 1 phase of the cell cycle, prior to the start of nuclear DNA replication. 4 Although numerous proteins have been functionally characterized and identified as vital components of kDNA replication and division, the molecular mechanisms governing this highly precise process remain largely unknown. 5 , 6 One division-related and morphologically characteristic structure that remains most enigmatic is the "nabelschnur," an undefined, filament-resembling structure observed by electron microscopy between segregating daughter kDNA networks. 7 , 8 , 9 To date, only one protein, TbLAP1, an M17 family leucyl aminopeptidase metalloprotease, is known to localize to the nabelschnur. 9 While screening proteins from the T. brucei MitoTag project, 10 we identified a previously uncharacterized protein with an mNeonGreen signal localizing to the kDNA as well as forming a point of connection between dividing kDNAs. Here, we demonstrate that this kDNA-associated protein, named TbNAB70, indeed localizes to the nabelschnur and plays an essential role in the segregation of newly replicated kDNAs and subsequent cytokinesis in T. brucei.
Competing Interests: Declaration of interests The authors declare no competing interests.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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