Direct extrusion of multifascicle prevascularized human skeletal muscle for volumetric muscle loss surgery.

Autor: Duong VT; Department of Electrical, Electronic and Computer Engineering, University of Ulsan, Ulsan, 44610, Republic of Korea; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA. Electronic address: thuydv1111@gmail.com., Dang TT; School of Biological Sciences, University of Ulsan, Ulsan, 44610, Republic of Korea; Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN 46202, USA. Electronic address: dangthaosphn@gmail.com., Le VP; Department of Electrical, Electronic and Computer Engineering, University of Ulsan, Ulsan, 44610, Republic of Korea. Electronic address: levbk03@gmail.com., Le TH; Department of Electrical, Electronic and Computer Engineering, University of Ulsan, Ulsan, 44610, Republic of Korea. Electronic address: lehuong94alhp@gmail.com., Nguyen CT; Department of Electrical, Electronic and Computer Engineering, University of Ulsan, Ulsan, 44610, Republic of Korea. Electronic address: nctrung1407@gmail.com., Phan HL; Department of Electrical, Electronic and Computer Engineering, University of Ulsan, Ulsan, 44610, Republic of Korea. Electronic address: phlam.ulsan@gmail.com., Seo J; Department of Electrical and Computer Engineering, Seoul National University, Seoul, 08826, Republic of Korea; Seoul National University Hospital Biomedical Research Institute, Seoul, 03080, Republic of Korea. Electronic address: callme@snu.ac.kr., Lin CC; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA. Electronic address: lin711@purdue.edu., Back SH; School of Biological Sciences, University of Ulsan, Ulsan, 44610, Republic of Korea; Basic-Clinical Convergence Research Institute, University of Ulsan, Ulsan, Republic of Korea. Electronic address: shback@ulsan.ac.kr., Koo KI; Department of Electrical, Electronic and Computer Engineering, University of Ulsan, Ulsan, 44610, Republic of Korea; Basic-Clinical Convergence Research Institute, University of Ulsan, Ulsan, Republic of Korea. Electronic address: kikoo@ulsan.ac.kr.
Jazyk: angličtina
Zdroj: Biomaterials [Biomaterials] 2025 Mar; Vol. 314, pp. 122840. Date of Electronic Publication: 2024 Sep 19.
DOI: 10.1016/j.biomaterials.2024.122840
Abstrakt: Skeletal muscle is composed of multiple fascicles, which are parallel bundles of muscle fibers surrounded by connective tissues that contain blood vessels and nerves. Here, we fabricated multifascicle human skeletal muscle scaffolds that mimic the natural structure of human skeletal muscle bundles using a seven-barrel nozzle. For the core material to form the fascicle structure, human skeletal myoblasts were encapsulated in Matrigel with calcium chloride. Meanwhile, the shell that plays a role as the connective tissue, human fibroblasts and human umbilical vein endothelial cells within a mixture of porcine muscle decellularized extracellular matrix and sodium alginate at a 95:5 ratio was used. We assessed four types of extruded scaffolds monolithic-monoculture (Mo-M), monolithic-coculture (Mo-C), multifascicle-monoculture (Mu-M), and multifascicle-coculture (Mu-C) to determine the structural effect of muscle mimicking scaffold. The Mu-C scaffold outperformed other scaffolds in cell proliferation, differentiation, vascularization, mechanical properties, and functionality. In an in vivo mouse model of volumetric muscle loss, the Mu-C scaffold effectively regenerated the tibialis anterior muscle defect, demonstrating its potential for volumetric muscle transplantation. Our nozzle will be further used to produce other volumetric functional tissues, such as tendons and peripheral nerves.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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