Development of a recombinant Lactobacillus plantarum oral vaccine expressing VP2 protein for preventing feline panleukopenia virus.

Autor: Li J; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Hongshan Laboratory, Wuhan, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China., Zeng Y; Wuhan Keqian Biology Co., Ltd, Wuhan, China., Li L; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Hongshan Laboratory, Wuhan, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China., Peng J; Wuhan Keqian Biology Co., Ltd, Wuhan, China., Yan Q; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Hongshan Laboratory, Wuhan, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China., Ye Z; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Hongshan Laboratory, Wuhan, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China., Zhang Y; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Hongshan Laboratory, Wuhan, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China., Li W; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Hongshan Laboratory, Wuhan, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China., Cao L; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Hongshan Laboratory, Wuhan, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China., Zhou D; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China., Li Q; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Hongshan Laboratory, Wuhan, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China., Si Y; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Hongshan Laboratory, Wuhan, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China. Electronic address: youhui@mail.hzau.edu.cn., Cao S; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Hongshan Laboratory, Wuhan, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China. Electronic address: sbcao@mail.hzau.edu.cn.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2024 Nov; Vol. 298, pp. 110257. Date of Electronic Publication: 2024 Sep 23.
DOI: 10.1016/j.vetmic.2024.110257
Abstrakt: Feline panleukopenia virus (FPV) represents a significant health threat to the kittens. While traditional vaccines administered via subcutaneous or intramuscular injection are effective, they can induce stress and adverse reactions. Moreover, unvaccinated kittens visiting veterinary clinics risk exposure to FPV, increasing their susceptibility to infection. Therefore, there is an urgent need for a safer, more gentle vaccination method with streamlined administration. In this study, we developed a recombinant L. plantarum NC8/VP2 expressing the VP2 protein of the prevalent Chinese FPV strain, FPV-251. Our results show that L. plantarum NC8/VP2 effectively colonizes the feline intestinal tract and induces high levels of neutralizing antibodies through oral administration. Kittens exhibited significant protection against FPV-251 infection and associated illnesses or fatalities after 30 days of continuous dosing. These results highlight the potential of recombinant L. plantarum NC8/VP2 as a novel oral vaccine for FPV, presenting a promising approach for disease prevention in domestic cats.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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