Superoxide dismutase positively regulates Cu/Zn toxicity tolerance in Sorghum bicolor by interacting with Cu chaperone for superoxide dismutase.

Autor: Jiang J; State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang, 550025, PR China., Zhang N; College of Agriculture, Guizhou University, Guiyang 550025, PR China., Srivastava AK; College of Agriculture, Guizhou University, Guiyang 550025, PR China., He G; College of Agriculture, Guizhou University, Guiyang 550025, PR China., Tai Z; College of Agriculture, Guizhou University, Guiyang 550025, PR China., Wang Z; College of Agriculture, Guizhou University, Guiyang 550025, PR China., Yang S; College of Agriculture, Guizhou University, Guiyang 550025, PR China. Electronic address: swyang@gzu.edu.cn., Xie X; College of Agriculture, Guizhou University, Guiyang 550025, PR China. Electronic address: ippxiexin@163.com., Li X; State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang, 550025, PR China. Electronic address: xyli1@gzu.edu.cn.
Jazyk: angličtina
Zdroj: Journal of hazardous materials [J Hazard Mater] 2024 Dec 05; Vol. 480, pp. 135828. Date of Electronic Publication: 2024 Sep 18.
DOI: 10.1016/j.jhazmat.2024.135828
Abstrakt: Heavy metal stress threatens plant growth and productivity. In this study, we investigated the effects of CuSO 4 and ZnSO 4 toxicity on sorghum seedlings, focusing on their impact on biomass, germination rates, growth parameters, antioxidant enzyme activities, gene expression profiles, and stress resistance mechanisms. As a result, eight sorghum superoxide dismutase (SOD) genes were identified, and their evolutionary relationships with cis-acting regulatory elements and their expressional patterns were evaluated. Integrating transcriptomic data revealed a key SOD member SbCSD1 that might contribute to plant abiotic stress resistance. Furthermore, SbCSD1 overexpression enhanced plant tolerance to CuSO 4 and ZnSO 4 stress by regulating SOD activity and interacting with copper chaperone for superoxide dismutase 1 (CCS1) in the plant nucleus and cytoplasm. Meanwhile, silencing CCS1 in SbCSD1-overexpressing plants revealed that SbCSD1 and CCS1 synergistically contribute to Cu stress tolerance. By integrating transcriptomic and genetic data, herein we provide novel insights into the orchestration of plant responses to heavy-metal stress in sorghum by SOD.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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