| Autor: |
Osaki T; Department of Molecular Patho-Biochemistry and Patho-Biology, School of Medicine, Yamagata University, Yamagata, Japan.; The Japanese Collaborative Research Group (JCRG) on Autoimmune Acquired Coagulation Factor Deficiencies Supported by the Japanese Ministry of Health, Labor and Welfare (MHLW), Yamagata University School of Medicine, Yamagata, Japan.; Department of Public Health and Hygiene, Yamagata University Graduate School of Medical Science, Yamagata, Japan., Magari Y; Q-may Laboratory Corporation, Oita, Japan., Souri M; Department of Molecular Patho-Biochemistry and Patho-Biology, School of Medicine, Yamagata University, Yamagata, Japan.; The Japanese Collaborative Research Group (JCRG) on Autoimmune Acquired Coagulation Factor Deficiencies Supported by the Japanese Ministry of Health, Labor and Welfare (MHLW), Yamagata University School of Medicine, Yamagata, Japan.; Department of Public Health and Hygiene, Yamagata University Graduate School of Medical Science, Yamagata, Japan., Ichinose A; Department of Molecular Patho-Biochemistry and Patho-Biology, School of Medicine, Yamagata University, Yamagata, Japan.; The Japanese Collaborative Research Group (JCRG) on Autoimmune Acquired Coagulation Factor Deficiencies Supported by the Japanese Ministry of Health, Labor and Welfare (MHLW), Yamagata University School of Medicine, Yamagata, Japan. |
| Abstrakt: |
Coagulation factor XIII (FXIII) is an enzyme that strengthens hemostatic clots, and its deficiency can cause life-threatening bleeding. We immunized mice with human plasma-derived FXIII to generate monoclonal antibodies (mAbs) against the B subunit (FXIII-B), which stabilizes the A subunit (FXIII-A) of FXIII, and analyzed their properties. The epitopes of the seven mouse antihuman FXIII-B mAbs obtained were found to be the 3rd, 5th, 6th, 9th, and 10th Sushi domains. One of these mAbs, mAb 5-6C, recognized the 10th Sushi domain and inhibited the fibrin cross-linking reaction without affecting the amine incorporation activity of FXIII. We previously reported that the 10th Sushi domain is the site where FXIII-B binds to fibrin and functions to bring FXIII-A closer to the substrate fibrin. Except for mAb 5-6C, mouse mAbs with high yields were used to measure the amount of FXIII-B antigen by an immunochromatography test (ICT), which showed a high correlation with enzyme-linked immunosorbent assay-obtained results. In addition, we developed a prototype ICT to detect anti-FXIII-B autoantibodies using mAb 1-3C, which showed good results in measuring the amount of FXIII-B antigen. Thus, mouse mAbs may be useful for clinical applications. mAb 5-6C targeting the 10th Sushi domain may also be useful for inhibiting thrombosis progression when humanized as antibody medicines. |
