Comparative analysis of the transcriptomes from regenerated plants and root explants of endangered Oplopanax elatus.

Autor: Seo JW; Interdisciplinary Program in Smart Science, Kangwon National University, Chuncheon, 24341, Republic of Korea., Choi HJ; Interdisciplinary Program in Smart Science, Kangwon National University, Chuncheon, 24341, Republic of Korea., Ham DY; Interdisciplinary Program in Smart Science, Kangwon National University, Chuncheon, 24341, Republic of Korea., Park J; Interdisciplinary Program in Smart Science, Kangwon National University, Chuncheon, 24341, Republic of Korea., Choi IY; Department of Agriculture and Life Industry, Kangwon National University, Chuncheon, 24341, Republic of Korea., Yu CY; Department of Applied Plant Sciences, Division of Bioresource Sciences, Kangwon National University, Chuncheon, 24341, Republic of Korea., Kim MJ; Department of Applied Plant Sciences, Division of Bioresource Sciences, Kangwon National University, Chuncheon, 24341, Republic of Korea., Seong ES; Department of Applied Plant Sciences, Division of Bioresource Sciences, Kangwon National University, Chuncheon, 24341, Republic of Korea. esseong@kangwon.ac.kr.
Jazyk: angličtina
Zdroj: Genes & genomics [Genes Genomics] 2024 Dec; Vol. 46 (12), pp. 1387-1398. Date of Electronic Publication: 2024 Sep 25.
DOI: 10.1007/s13258-024-01566-y
Abstrakt: Background: Oplopanax elatus is a plant of therapeutic significance in oriental medicine; however, its mass cultivation is limited owing to the difficulties in propagating it from seeds.
Methods: In this study, we investigated the transcriptome profiles and transcriptional regulatory factors expressed during plantlet regeneration from root tissues of the endangered O. elatus.
Results: The RNA-seq results for the control and regenerated plants cultured in liquid medium for 8 weeks showed that the clean length of the control group was 11,901,667,912 and that of the 8-week sample was 10,115,155,171, indicating a clean value of 97% for both samples. The number of mapped paired-end reads was 63,922,480 for the control group and 54,146,902 for the 8-week sample. The number of genes for which at least one clean data point was mapped was 43,177 in the control group and 42,970 in the 8-week sample. The results of the differentially expressed gene analysis indicate that the number of upregulated genes in the 8-week sample was 158, and the number of downregulated genes was 424. Gene Ontology (GO) analysis of the upregulated genes revealed that GO terms were classified into 14 categories, and genes expressed in the biological process category occurred most frequently. GO terms of the downregulated genes were evenly distributed into two categories: biological process and molecular function. From the upregulated genes, eight reference genes with significant differences in expression were selected and analyzed using real-time PCR. The Oe38836 gene (late embryogenesis abundant protein M17-like isoform X1) showed the highest expression rate that was more than tenfold that of the control. Oe40610 (auxin-responsive protein SAUR21-like) and Oe07114 (glucose-1-phosphate adenyl transferase-like protein) genes showed expression levels that were increased eightfold relative to the control.
Conclusions: The RNA sequencing (RNA-seq) results from the plants regenerated through liquid culture of O. elatus root tissue were confirmed using real-time PCR, indicating their reliability.
Competing Interests: Declarations. Conflict of interest: The authors declare no competing interests.
(© 2024. The Author(s) under exclusive licence to The Genetics Society of Korea.)
Databáze: MEDLINE
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