A longevity-specific bank of induced pluripotent stem cells from centenarians and their offspring.

Autor: Dowrey TW; Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, Massachusetts, USA.; Section of Hematology and Medical Oncology, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts, USA., Cranston SF; Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, Massachusetts, USA.; Section of Hematology and Medical Oncology, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts, USA., Skvir N; Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, Massachusetts, USA.; Section of Hematology and Medical Oncology, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts, USA., Lok Y; Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, Massachusetts, USA.; Section of Hematology and Medical Oncology, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts, USA., Gould B; Section of Geriatrics, Department of Medicine, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts, USA., Petrowitz B; Section of Geriatrics, Department of Medicine, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts, USA., Villar D; Albert Einstein College of Medicine, Bronx, New York, USA., Shan J; Albert Einstein College of Medicine, Bronx, New York, USA., James M; Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, Massachusetts, USA., Dodge M; Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, Massachusetts, USA., Belkina AC; Flow Cytometry Core Facility, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts, USA.; Department of Pathology and Laboratory Medicine, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts, USA., Giadone RM; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts, USA., Milman S; Albert Einstein College of Medicine, Bronx, New York, USA., Sebastiani P; Institute for Clinical Research and Health Policy Studies, Tufts Medical Center, Boston, Massachusetts, USA., Perls TT; Section of Geriatrics, Department of Medicine, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts, USA., Andersen SL; Section of Geriatrics, Department of Medicine, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts, USA., Murphy GJ; Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, Massachusetts, USA.; Section of Hematology and Medical Oncology, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts, USA.
Jazyk: angličtina
Zdroj: Aging cell [Aging Cell] 2024 Sep 25, pp. e14351. Date of Electronic Publication: 2024 Sep 25.
DOI: 10.1111/acel.14351
Abstrakt: Centenarians provide a unique lens through which to study longevity, healthy aging, and resiliency. Moreover, models of human aging and resilience to disease that allow for the testing of potential interventions are virtually non-existent. We obtained and characterized over 96 centenarian and offspring peripheral blood samples including those connected to functional independence data highlighting resistance to disability and cognitive impairment. Targeted methylation arrays were used in molecular aging clocks to compare and contrast differences between biological and chronological age in these specialized subjects. Isolated peripheral blood mononuclear cells (PBMCs) from 20 of these subjects were then successfully reprogrammed into high-quality induced pluripotent stem cell (iPSC) lines which were functionally characterized for pluripotency, genomic stability, and the ability to undergo directed differentiation. The result of this work is a one-of-a-kind resource for studies of human longevity and resilience that can fuel the discovery and validation of novel therapeutics for aging-related disease.
(© 2024 The Author(s). Aging Cell published by Anatomical Society and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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